Study On Removal Of Hydrogen Sulfide By Immobilization Of Thiobacillus Ferrooxidans

Along with the strength of environmental protection regulation and day by day requiring strictness of environmental protection,the development research of biodesulfurization technique has become the issue solved forwardly in environmental protection field. In this assignment,we use the indirect oxidation of Thiobacillus ferrooxidans(ab.T.f)We augmented the cell density and accelerated the reaction by immobilization technique,then the continuous operation was realized and the upper work capacity was obtained.In this thesis we studied parts of removing H2S in acidic industrial gas using T.f from bacterium,reactor and absorption tower.In this thesis the T.f conserved in our laboratory was filtered and mutagenized in lower pH1.8.The maximum oxidation rate of Fe2+ was 0.404g/lh as more as 28.6% before mutagenized.The best condition of culture of T.f was the rotate speed is 150r/min,temperature is 30℃,liquid quantum is 100ml.We studied the optimum concentration of culture of mutagenized T.f using RSM and the growth dynamics model. The optimum concentration of culture is (NH4)2SO4 3.1296g/l,K2HPO 40.5006 g/l,MgSO4 0.5059 g/1.Using mutagenized T.f, we confirmed the optimum condition of immobilization. It is pH1.8 and temperature 30℃.The bacterium of absorption in carriers is more and the oxidation rate of Fe2+ increased 32.37% than before immobilization. The precipitation was decreased and this profited the continue operation of bioreactor.We construced the fixed bio-reactor with H-2 soft filling as carrier and examined the influence of aeratin rate, dilution and the concentration of reactant and product in the culture medium andobtained the dynamics model of the fix bed bioreactor.We studied the removal of H2S in acidic industrial gas and studied the effects of liquid gas ratio,the concentration of H2S and temperature on the abatement efficiency of H2S.We obtained the apparent kinetic equation.We studied the adjustment of 9K reverting culture and we can realize continue and circulate operation of the bioreactor before adjusted the pH and added right 9K culture.