Study On The Application Of Solid Phase Extraction Technique In Some Kinds Of Important Tobacco Components Analysis

The application of solid phase extraction technique in some kinds of important tobacco components analysis was studied. Four new analytical method systems 'The determination of tobacco leaf pigments by solid phase extraction and high performance liquid chromatography', 'The determination of polyphenols in tobacco by solid phase extraction and high performance liquid chromatography coulped to mass spectrometry', 'The determination of sugar in tobacco and the nonvolatility components in tobacco sauce by solid phase extraction and high performance liquid chromatography', and 'The determination of iron, cobalt, nickel, copper, zinc and manganese ions in tobacco by solid phase extraction and the reversed-phase high-performance liquid chromatography by using 2-(2-quinolinylazo)-5-dimethylaminophenol as pre-column derivatization regent' were established.(1) The determination of tobacco leaf pigments by solid phase extraction and high performance liquid chromatographyA high performance liquid chromatography (HPLC) method for the determination of tobacco leaf pigments in tobacco was studied. The tobacco leaf pigments were extracted from tobacco leaf with 90% acetone by oscillating extraction, then subjected to be separated and enriched by solid phase extraction with Sep-Pak-C18 cartridge. The chlorophyll-a,chlorophyll-b, (-carotene, lutein, neoxanthin, violaxanthin, pheophorbide and other tobacco leaf pigments can be separated on a Nova-Pak-C18 (3.9(150 mm,5 (m) chromatographic column, with methanol-isopropyl alcohol-water gradient elution as mobile phase at a flow-rate of 0.5 ml/min, and detected with photodiode array detector. The recoveries of tobacco leaf pigments are 92%(101%, and the relative standard deviations are 1.24%(2.31%. This method was applied to determine pigments in tobacco leaf with satisfactory results.(2) The determination of polyphenols in tobacco by solid phase extraction and high performance liquid chromatography coulped to mass spectrometryA high performance liquid chromatography (HPLC) and mass spectrometry method for the determination of polyphenols in tobacco was studied. The polyphenols were extracted from tobacco sample with 80% of methanol by heating circumfluence, then subjected to degrease by solid phase extraction with Waters Sep-Pak-C18 cartradge. The chlorogenic acid, rutin, scopoletin, caffeic acid, scopolin and other polyphenols were separated on a Waters Nova-Pak-C18 chromatographic column (3.9×150 mm,5 (m), with methanol and 0.05 mol/l potassium dihydrogen phosphate buffer solution as mobile phase at a flow-rate of 0.5ml/min, and detected with photodiode array detector. The recoveries of polyphenols are 95%(105%, and relative standard deviations are 1.45%(1.85%. This method was applied to the determination of polyphenols in tobacco with satisfactory results.(3) The determination of sugar in tobacco and nonvolatility in tobacco sauce by solid phase extraction and high performance liquid chromatographyA high performance liquid chromatography (HPLC) method for the determination sugar in tobacco was studied. The sugars were extracted from tobacco with water and purified by solid phase extraction with Sep-Pak-C18 cartridge. Sucrose, maltose, glucose, lactose and fructose were separated on a Waters Carbohydrate column(6.5(300 mm, 4 (m), with 75% acetonitrile solution as mobile phase and detected with a refractive index detector system. A high performance liquid chromatography (HPLC) method for the determination of sugars, glycerol, propylene glycol and xylitol in tobacco sauce was studied. The sugars, glycerol, propylene glycol and xylitol were separated on a Waters Suger-Pak-I cation ion exchange column (6.5×300 mm, 8 (m), with 0.05 g/l EDTA calcium disodium salt solution as mobile phase and detected with a refractive index detector system. The relative standard deviations were 0.62%～1.2%, and the standard recoveries were 98～102%. This method was applied to the simultaneous determination of sugars, glycerol, propylene glycol and xylitol in tobacco and tobacc...