Study On Improving The Activity Of Anthracene Degrading Bacteria With Surfactant Existence

Polycyclic Aromatic Hydrocarbons (PAHs) --a kind of toxic organic contamination widely distributed in the environment— is difficult to be degraded by biology. PAHs in the environment can be absorbed into the organism and sediment easily and enter into body of human through the food chain. It can do a great potential harm to human health and the environment. Bioremediation is an effective method of treating PAHs contamination. The An815 has a high capability of degrading PAHs and ZW25 can use surfactant Tw-80 preferably. By means of cell electrofusion, this experiment is expected to get the Genetic Recombination Microorganisms (GRMs) which has more capability for degrading PAHs. The whole work includes:1. By studying the different biodegrading effect of the different surfactants (Tw-80, SDS, LAS) on anthracene, TW-80 was choosed, which has lower toxicity but stronger solubilization, to enhance the water solubility.2. A bacterium, named ZW25, which can effectively degrade TW-80 isolated from contaminated soil and preliminarily identified as Pseudomonas sp. The optimum living condition is pH6.5, 30°C.The bacterium can grow very well even under the concentration TW-80 of 5000mg/L.3. Conditions for the preparation and regeneration of protoplast of ZW25 and An815 were studied by cross-test experiments. Effect level of different factors was discussed and the optimum condition was confirmed.4. Though protoplasts of An815 and ZW25 electrofusion, the fusant AT13 was gained, which not only had the capability of degrading Polycyclic Aromatic Hydrocarbons of An815 but also can use surfactant (Tw-80) as the carbon resource. The capability of degrading Polycyclic Aromatic Hydrocarbons was improved greatly.