Study On Construction Of Bioaugmentation Degradative System And Degradation Capability For Hydrophobic Hydrocarbon Degrading Bacteria

Environmental problem caused by petroleum and its product have fallen under observation increasingly. Based on petroleum pollution status and environmental requirements, low expensive and high efficient bioaugmentation technology possesses strongly industrial feasibility. If this technology can successfully be used for the disposal of petroleum pollution, it's sure to bring forth huge social benefit and economic benefit. Bioaugmentation technology is one significant means to harness petroleum hydrocarbon contaminant in the bioremediation fields. Due to the complicated composition of petroleum, treatment efficiency of petroleum pollution wastewater isn't high. It should be lucubrated of certain pollutant on degradation property, flexibility in environment, cost benefit, and so on. As petroleum hydrocarbon pollutants are primarily hydrophobic compounds, with the guidance of bioaugmentation, three high efficient hydrocarbon degrading bacteria named HDB-1,HDB-2, HDB-3 were separated from the oil contaminant soil, with hydrophobic material polyurethane foam(PUF) as bio-carrier, degraded artificial-simulated petroleum pollution wastewater in the reactor. The primary contents included several aspects as following.1. Three high efficient hydrocarbon degrading bacteria named HDB-1, HDB-2, HDB-3 were separated from the oil contaminant soil. Observing their growth status, bacterial colonies characteristic, bacterial shape and so on, bacterial species were ascertained.2. Studied the nutrient balance and mechanism for enhancing the bioremediation of petroleum contaminated wastewater with bacteria HDB-1 in inorganic salt solution of 1000mg/L oil content. Using nitrogen source and phosphorus source respectively, and researched use effect of some representative inorganic nitrogen source (include ammonium salt and nitrate) and organic nitrogen source, the equilibrium relationship of nitrogen phosphorus element and the effect on degradation ratio.3. Studies on the cell-surface hydrophobicity of HDB-1,HDB-2, HDB-3 and environment influence factor by MATH offered new theoretic reference for petroleum contaminated bioremediation of high efficient hydrocarbon degrading bacteria.4. On the precondition of adequate oxygen supply and nitrogen phosphorus nutritive equilibrium, through perpendicular experiment, the optimum substrate concentration and growth temperature and pH were ascertained. The major factors to impact degradation rate were also ascertained through range analysis.5. Choosing polyurethane foam (PUF) as bio-carrier, its absorptive capability was tested by oil and water absorption test. After pretreatment of chemical, the foams were thrown into the biological system as suspended carrier.6. High efficient hydrocarbon degrading bacteria were thrown into suspended-carrier biological reactor, and the indices of COD> NH3-N were inspected. The experiment results showed throwing suspended carrier into aeration basin not only enhanced activated sludge concentration, but also improved living environment of degrading bacteria. It proved that this process was feasible to dispose petroleum pollution wastewater.