The Research On Multiresidue Determination Of Fluoroquinolones In Pig Tissue By HPLC

The thesis consists of two parts. The first part analytical method of fluoroquinolones residues in esculent animals are introduced. In this paper pork is the analytical object and SPE-HPLC methods of determination of fluoroquinolones multiresidues are summarized. In the second part analytical method of determination of salvianolic acid in yangxinshengmai granule is introduced. The details are as following:In Chapter One: actuality and progress technologies of determination of common pharmaceutical fluoroquinolones are summarized. Firstly, introduced progress on analytical method of fluoroquinolones. Secondly, summarized determination technology on fluoroquinolones residues. Finally, showed some examples to introduce how to analyze fluoroquinolones residues in esculent animal samples.In Chapter Two: pork is the analytical object in this paper. The determination of fluoroquinolones multiresidues in solid animal samples are introduced. The samples is extracted by acetonitrile-acetic acid, then extracted by n-hexane,then purified by solid phase extraction C18 cartridge, finally determined by HPLC. The linear range of the method is 0.1～10 mg/L; the detection limit is 9～14 μg/kg; the recovery of the method is 66～92% (RSD<5%, n=3) .The parameters of high performance liquid chromatography (HPLC)-diode array detector (DAD) are confirmed. The mobile phase is confirmed by regulation of the component of buffer solution and the proportion of organic solvent and water, and many fluoroquinolones are separated excellently. Then each fluoroquinolone is determined by retention time and the max absorption wavelength. The external standard method is used, and the accuracy, precision, linear regression, detect limit are validated.The method can be used to determine seven fluoroquinolones in the esculent animal samples at the same time with high accuracy, high precision, good linear relationship and low detect limit.