Study On The Biotoxicity Of Reactive Brilliant Red X-3B And NaCl On Phanerochaete Chrysosporium

White rot fungi are capable of degrading many kinds of dyes wastewater. During the degrading process, the dyes wastewater may have an adverse influence on the cellular ultrastructure, isoenzyme peroxidase production. Also the DNA may be damaged. The analysis of the toxic effects provided a method for the further study in the field of biodegradation of dyes wastewater.According to the experimentation analysis, the results are as follows:1. The biotoxicity of Reactive Brilliant Red X-3B (RBR X-3B) on the cells of Phanerochaete chrysosporium was studied. After exposure to RBR X-3B for 10 days, the ultrastructure of hyphal cells were evidently damaged. At the concentration of 100 mg/L, RBR X-3B induced plasma membrane separating from cellular wall. The above adverse effects became more evident with the increase of RBR X-3B concentration. NaCl had the same influence on the cells of Phanerochaete chrysosporium2. Isoenzyme patterns of peroxidase of Phanerochaete chrysosporium under different dye or salt concentrations were investigated. Lower concentrations of RBR X-3B or NaCl could obviously induced over-production of peroxidase, whereas high concentrations of RBR X-3B (200mg/L or even higher) had greatly inhibitive effects on peroxidase production. The results indicated Phanerochaete chrysosporium had undergone a strong response for peroxidase production to dye and salt concentrations, that was inducement, inhibition and exhaustion.3. Amplified Fragment Length Polymorphism(AFLP) results generated many clear bands. When the concentration of RBR X-3B less than 400 mg/L, some new bands could be obtained. In the range of high concentration (500 mg/L), the damage of DNA was remarkable. NaCl could also induce damage to DNA, and the effect enhanced with the increase of NaCl concentration.4. The AFLP system suitable for Phanerochaete chrysosporium was developed, clear and high resolution bands were obtained. The developed techniques could be applied in the analysis of genetic damage.5. The spectrum was characterized as two groups of triplet set of doublet lines in nitrogen using phenyl-tert-butyl nitrone(PBN) ,solved in dimethylsulphoxide(DMSO), as trapping agent. But the signal could not be detected.