Roles Of Oxidative Stress And Mitochondrion In Cadmium-induced Injury Of Liver Cells

With the fast development of industry, environment pollution has been more and more serious. The mechanisms of toxic effect on cadmium, as a heavy metal with high toxicity, attracted many scientists to study. At present, most studies are focus on the impairment of oxidative stress and mitochondrion induced. The aim of this study is to analyze the roles and relationship of oxidative stress and mitochondrion in cadmium-induced injury.In this experiment, SD-rats were used as experimental material. Through acute exposure to cadmium, the two mechanisms were studied by using NAC and ALCAR. The experimental indexes were chosen including MMP, total amounts of ROS, content of GSH, activity of GSH-Px, content of MDA and DNA single-strand break.NAC or ALCAR was administrated, prior to Cd exposure. The treatments were divided into four groups: rats treated with Cd, or first with NAC or ALCAR and then with Cd, or only injected with physiological saline. The results are as follows:Comparative analysis showed that the MMP of all exposure groups were decreased significantly, group NAC< group Cd< group ALCAR. NAC can enhance the injury of mitochondrion by cadmium-induced. On the other hand, ALCAR can play a protective role.In all exposure groups, the ROS level was higher than that of group CT, group NAC