Synthesis Of Noble Metal Nanoparticles And Research On Their Catalytic Properties On Chemiluminescence And PCR

About two thirds of elements on earth are metal elements. The properties of metal nanoparticles are always the hotspots in research field. Core-shell structure compound nanoparticles play a significant role in both theoretical research and application. Nanoparticles synthesized by different metals not only meliorate the problem that nanoparticles consisting of only one kind of metal will agglomerate, but also possess many kinds of functions. Among these functions, catalytic property of metal nanoparticles, especially noble metal nanoparticles is widely used in various kinds of fields. In this dissertation, we mainly focus on the stud on the preparation of metal nanoparticles and their catalytic ability.We prepare Fe3O4 nanoparticles by co-precipitation method and reduce their surface particles into Fe by reducing agent. Water-soluble Fe3O4@Ag core-shell nanoparticles are synthesized by the replacement reaction of Ag+ and Fe. We character the nanoparticles by UV-vis, particle size analyzer, TEM, and XRD. We investigate the effect of reactant concentration ratio on the morphology of the compound nanoparticles. The synthesis method is relatively simple and low-cost. The Fe3O4@Ag core-shell nanoparticles stabilized by citric acid radical are well dispersed in water phase and they display magnetic and catalytic property, as well as good biocompatibility. Consequently, they will have a bright future in biological application.The silver nanoparticles (AgNPs) with different diameters are prepared by chemical reduction method. We investigate the influence of AgNPs on chemiluminescence of luminol-H2O2 system and find AgNPs can enhance the intensity of chemiluminescence of luminol-H2O2 system. We also investigate the effects of diameters of AgNPs, pH, and concentration of reactants on chemiluminescence. AgNPs are bound with thioglycolic acid (Ag-HSCH2COOH) and then joined to BSA protein (Ag-BSA). We study the effect of Ag-HSCH2COOH and Ag-BSA on the intensity of chemiluminescence and elucidate the possible mechanism of the catalytic reaction.We also study on the effect of noble metal nanoparticles on polymerase chain reaction (PCR). We add silver and platinum nanoparticles into the reaction and investigate the effect by adjusting the concentration of DNA template and the annealing temperature. We find the catalytic property of platinum on PCR is better than that of silver. Platinum nanoparticles within a certain range of concentration exhibit the enhancement on PCR and improve the sensitivity of PCR when the DNA template concentration is relatively low. The mechanism of the enhancement requires further research.