| Nanomaterials are high-tech products in the21st century. Nanomaterials haveattracted great interest because of its special physical and chemical properties.Nanomaterials have been applied in many fields including environmental monitoring,biomedical, home appliances, textile, mechanical and so on. Though chemiluminescence（CL） has been studied for many years, it was limited to molecular and ion levels.Recently, more and more attentions have been extended to liquid-phase CL systemsparticipated by metal nanomaterials, because of their unique properties. In this paper,metal nanoparticles （gold, silver） are used as catalyst to design new CL detection systems.The applications of these new CL detection systems to analysis of medicine, biologicaland environmental samples are described.In this thesis, the review involves the concepts, development, principles andcharacteristics of chemiluminescence and chemiluminescent reagent in common use, thechemical and physical properties of metallic nanomaterials and their applications in CLanalysis. Furthermore, silver nanoparticles （AgNPs） and gold nanoparticles （AuNPs） areutilized in liquid-phase CL systems. Suh as AgNPs catalysed luminol-K3Fe（CN）6systemfor the determination of paracetamol and bovine serum albumin（BSA）, AgNPs catalysedluminol-KMnO4system for the determination of bisphenol A（BPA）, AgNPs catalysedluminol-KIO4system for the determination of indapamide and AuNPs catalysedluminol-AgNO3system for determination of nonylphenol （NP）. The main contents are asfollows:1. The experiments indicated that silver nanoparticles （AgNPs） could greatlyenhance the chemiluminesence intensity of the luminol-K3Fe（CN）6system in sodiumhydroxide media, but paracetamol could inhibit the chemiluminesence signalssignificantly. The experimental conditions that influence the chemiluminescenceintensity were carefully studied and optimized. Under the optimum conditions, thecalibration graph for paracetamol was obtained. The linear range for the determination ofparacetamol was9×10-12to1.0×10-10g/mL （0.9947） and1.0×10-10to7.0×10-9g/mL（0.9904） with a detection limit of7.9×10-12g/mL. The relative standard deviations （RSD） for11repeated measurements of5.0×10-11g/mL and5.0×10-10g/mL paracetamol were1.3%and1.3%, respectively. Possible interferences from major excipients inpharmaceuticals and other related compounds were also studied. Contents of paracetamolin two locally available pharmaceuticals were analyzed. The method had the merits ofrapid, simple, high sensitivity, wide linear range and has been used for the analysis ofparacetamol in commercial tablets with recoveries in the range from97.0%to101.5%.2. In the first time, silver nanoparticles were used in luminol-KIO4CL system andalso studied its chemiluminescence mechanism. It was found that silver nanoparticlescould enhance the CL intensity of the luminol-KIO4system in sodium hydroxide mediumand indapamide could greatly inhibit this enhanced CL intensity. Based on theobservations, coupled with flow-injection technology, a novel flow-injection CL methodfor the determination of indapamide has been established. Under the optimizedconditions, the linear range for the determination of indapamide was1.0×10-9to2.0×10-8g/mL （0.9941） and2.0×10-8to1.0×10-7g/mL （0.9978） with a detection limit of6.0×10-10g/mL. The relative standard deviations （RSD） for11repeated measurements of5.0×10-9g/mL and5.0×10-8g/mL indapamide were2.1%and1.8%, respectively. Themethod has been successfully applied to the determination of indapamide in tablets withrecoveries in the range from97.0%to102.0%.3. A simple, sensitive, and rapid flow-injection chemiluminescence method wasreported for the determination of bovine serum albumin （BSA）. The method was basedon the fact that the silver nanoparticles （AgNPs） enhanced the chemiluminescence of theluminol-K3Fe（CN）6system and that BSA inhibited the CL signals in alkaline solutionobviously. The experimental conditions that influence the CL intensity were carefullystudied and optimized. CL spectra, UV-visible spectra were used to investigate themechanism of the CL reaction system. Possible interferences were also studied.Under theoptimum conditions, the relative CL intensity was correlated linear with theconcentration of BSA over the range of2.0×10-102.0×10-8mol/L and the detection limit（3） was8.9×10-11mol/L. The method had been successfully applied to the determinationof BSA in milk samples.4. Silver nanoparticles （AgNPs） were found to enhance the CL intensity of theluminol-KMnO4system, and bisphenol A could inhibit this CL signals in the medium ofsodium hydroxide. Based on this inhibitive phenomenon, the determination of bisphenolA in plastic products has been established by flow injection CL method. Under theoptimized conditions, the linear range for the determination of bisphenol A was8.0×10(-11 to1.0×10-8g·mL-1（0.9926） and1.0×10-8to8.0×10-8g/mL （0.9916） with a detection limitof6.5×10-11g/mL. The relative standard deviations（RSD） for11repeated measurementsof5.0×10-10g·mL-1and5.0×10-8g/mL bisphenol A were1.4%and1.0%, respectively. Themethod has been successfully applied for determining of bisphenol A in plastic productswith recoveries in the range from97.0%to105.0%.5. A novel, quantitative analytical method for measuring nonylphenol （NP） has beendeveloped based on the catalytic activity of gold nanoparticles （GNPs） andluminol-AgNO3system for the first time. It has been found that gold nanoparticles（AuNPs） enhanced the CL intensity of the luminol-AgNO3system and that nonylphenol（NP） inhibit these CL signals in alkaline solution. Under the optimized conditions, thelinear range for the determination of NP was3.0×10-8to3.0×10-6g/mL. The detectionlimit was1.2×10-8g/mL. The relative standard deviations（RSD） for the determination of1.0×10-7g/mL NP was2.8%（n=11）. This proposed method has been used for thedetermination of NP in real water samples. |
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