Study On The Properties Of The Pigments Produced By A Bacterium And The Identification Of The Strain

The pigment includes natural pigment and synthetic pigment. In recent years, Sudan, malachite green, and other commonly used synthetic pigment incidents also caused a lot of food safety alerts, which the people of pigment security concerns have gradually deepened. But natural pigments has continuously demonstrated its own beneficial to human health of the many unique advantages: most natural pigment from edible plants,animals and microorganisms, safe and non-toxic; many natural pigments contain the necessary nutrients to human or itself is a vitamin or the material with vitamin natures; some natural pigments have pharmacological effects and can treat some diseases; natural pigment colors are natural, can be easily accepted, and some also have nice smell, added to the food will bring a happy feeling. Therefore, more and more people like to use the natural pigments. However, the natural pigment also has its own shortcomings: on the one hand, the natural pigment has poor stability and expensive; on the other hand, most of the natural pigment today used is been extracted from plant tissue, at the same time the planting is impacted by the climate and occupies lots of land, which Restrict its Extensive use. Developments of new sources of natural pigments, in particularly, the use of microbes to produce natural pigments have caused widespread concerns.In this work, a bacteria which can produce green pigment isolated from soil is studied. First of all, conditions of the strain to produce the pigment were explored and found that potato medium, static culture, glucose or sucrose choose carbon, nitrogen choose yeast (or cream), culture conditions for the temperature of 28℃, pH about 6, the culture time about 72 h, the stain will produce the largest pigment. Secondly, The extraction and separation of the pigment were studied, we have used organic solvent extraction,activated carbon adsorption,paper chromatography and other methods, this pigment is water-soluble and can not dissolve in most organic solvents, we extract two kinds of pigments: the blue one and the yellow one. Both of the pigments extracted can not be easily damaged by the high temperature,pH,light,oxidants and reducing agents,metal ions factors. Finally, we do some physiological and biochemical experiments and 16SrRNA identification to this strain .