Study On Preparation Of Novel Composite Carrier And Its Application In Immobilization Of Microbes For High Concentrations Of Phenol Treatment

This dissertation reviews microorganism immobilization and two-phase partitioning bioreactor was used to degrade high concentrations of phenol. Based on the current situation and existing problems, the detailed research progress is presented.Foam carrier based on composite of polyurethane/montmorillonite (PU/OMMT) was designed and prepared successfully, By addition of organic modified montmorillonite (OMMT) to polyurethane (PU) foam, the chemical stability and bio-stability of PU have been improved. Accordingly, the addition of organic modified montmorillonite has increased the lifetime of PU. The composite PU was analyzed by SEM and its chemical and biological stability was examined. It was found that the organic modified montmorillonite could get perfect performance when the concentration of organic modified montmorillonite is 1%.The high efficiency microbes B350 were immobilized on the PUF/OMMT. The effects of initial phenol concentration, pH and temperature on the removal of phenol were investigated. Comparing with free microbes, we found that phenol-stability and degradative efficiency of immobilized microorganism had been improved, which indicate that immobilized microorganism has the obvious character that is not affected by environmental factor easily. The dynamical mode which the immobilized microorganism degrades phenol was built, and then, the kinetic parameters were determined by non-linear curve fit, at last, the dynamical mode was check by experimental data, the result showed that the dynamical mode was accorded with the experimental data. The dynamical mode provided theory for the application which the immobilized microorganism degrades phenol.The polymers of polysulfone/nonanol and polysulfone/OMMT were prepared using the phase inversion method, the capability of two polymers were tested individually, the results show that two polymers all have fast sorption velocity: polysulfone/nonanol capsule can reach sorption balance within one hour, and polysulfone/OMMT bead used two hours; Two polymers also have high sorption capacity: the sorption capacity of polysulfone/nonanol capsule is 16.3mg/g, when the initial phenol concentration is 1906mg/L; the sorption capacity of polysulfone/OMMT bead is 30.2mg/g, when the initial phenol concentration is 2030mg/L. The adsorption dynamical mode of polysulfone/OMMT bead accords with Lagergren pseudo-second-order adsorption model. The adsorption to phenol also accords to Freundlich adsorption model.Two-phase partitioning bioreactor containing immobilized microorganism and PS/OMMT bead was used to degrade high concentrations of phenol, of which the degradation velocity is 342.4 mg/L*h, while, two-phase partitioning bioreactor containing free microorganism and PS/OMMT bead was used to degrade high concentrations of phenol, of which the degradation velocity is 208.4 mg/L*h. The degradation velocity of phenol and initial phenol concentration were improved, because TPPB contains immobilized microorganism.TPPB was made up of immobilized microorganism used as the phase of biology and PS/OMMT bead used as the phase of extraction, which was used to degrade high concentrations of phenol. That is the innovation of this thesis.