Biohydrogen Production From Simulated Starch Wastewater By Anaerobic Fermentative And Fuel Cell Electricity Generation Integration

Bio-hydrogen production from anaerobic fermentation, taking the organic waste as raw material, using microorganism own metabolism, decomposes the substrate and products hydrogen under comparative moderate condition. It can realize converting waste into resources, and the cost is inexpensive. It is environment friendly and has a broad prospects to product hydrogen. Coupling bio-hydrogen production from anaerobic fermentation to proton exchange membrane fuel cell electricity generation directly, it could be achieved the conversion of waste to electricity. This study includes two aspects work. Firstly, sewage sludge and pure bacterium used as bacterium resources in the process of simulative starch wastewater anaerobic fermentation, separately. Realized the dual aim of biohydrogen production and starch wastewater degradation. Through PEMFC, hydrogen converted to electric power. Secondly, sewage sludge decreases by ultrasonic.Mostly study work as follow:1. The sewage sludge taken from a municipal wastewater treatment plant, without pretreatment, worked as bacteria resource. When the reaction temperature was 37℃, the optimal pH and substrate concentration were 6.5, 5g/L, respectively. Under optimal conditions, the maximum hydrogen yield was 186mL/g-starch and the hydrogen content was 75.4%. Methane was also detected in the biogas. The maximum biogas production rate was 220mL/h. Biogas produced from 300mL simulative starch wastewater anaerobic fermentation could drive the fuel cell and generate 190J power.2. Klebsiella pneumoniae was also used as bacteria resource. The optimal volume of bacterium, initial pH and substrate concentration were 24mL, 7.0, 1g/L at 37℃, respectively. Under optimal conditions, the maximum hydrogen yield was 192mL/g-starch and the hydrogen content was 65.4%. There were only hydrogen and carbon dioxide detected in the biogas, no other kind of gas was produced.3. Parallel experiments were conducted to examine the effect of ultrasonic time on COD and SCOD value of sewage sludge. The result shows that, under definite sound intensity and sound energy density, COD value decreased as the ultrasonic time prolonged. Within definite time, SCOD value increased as the ultrasonic time prolonged. When the time continue prolonged, the increased rate of SCOD was decreased, didn't continue the linearity increase.Compared two groups experiment result of simulate starch wastewater anaerobic fermentation, it can be found that when used the mixed microbial in sewage sludge as bacteria resource, the operation was simple, and also obtained the high substrate conversion rate and the high hydrogen content.