The Separation Of Effective Components In Natural Products Using Molecular Imprinting Technology

Molecular imprinting technology(MIT) is a kind of new-emerging polymerizing technology and is receiving extensive attention in some fields. Molecular imprinted polymers (MIPs) have been applied in the field of separation materials, artificial antibodies, enzyme mimic catalyst, mimic biosensors etc. At present, an idea of the hoping of"returning to nature"and"non-pollution"consumption is recognized. Extraction of effective components from natural products is becoming a tendency and exhibits a profound significances to social and economic development. To exploit a new and high efficient extraction method of effective components from natural products is very important. The application of molecular imprinting technology to the separation of luteolin from peanut hull is studied.The molecular imprinted polymers (MIPs) are prepared by using Luteolin as the template molecular, acrylamide as the functional monomer, EDMA as the crosslinking agent in acetone. The molecular imprinted polymers are investigated in equilibrium binding experiments to evaluate its molecular recognition and binding characteristics It exhibits a very high selectivity and good recognition ability to Luteolin. The influence of several parameters on the resolution of Luteolin is investigated, including the amount of functional and cross-linking monomers, the molar ratio of the target molecule to the monomers and so on. Adsorption isotherms are obtained with staticadsorption method. Then the Scatchard analysis shows that the molecularly imprinted polymers using acrylamide as functional monomer could form two kinds of non-equivalent binding sites. The dissociation constants of MIPs are estimated to be K d1=4.1×10-5mol/L, K d2=5.8×10-4mol/L. The most apparent calculated adsorption capacities are about Qm ax1=17.49μmol/g; Qm ax2= 63.99μmol/g respectively.An ultrasonicwave-assisted solvent extraction method has been developed for luteolin from peanut hull and the crude ratio is 3.82%. The application of the imprinted polymers to the separation of Luteolin from complicated samples(leachate of peanut hull) and the influences of washing solvents, eluting solvents and flow rate are studied. Luteolin in leachate of peanut hull is extracted by MIPs, the results show that it can be preconcentrationed and eluted effectively with the recovery of 98.0%. The packings showed good stability with the recovery of 94.7% after being used for10 times. Moreover, through the methods of TLC, UV spectra and IR appraise the separate product.The application of MIP-SPE to separate Luteolin from leachate of peanut hull provides a new route for the separation and purification of effective components from natural products.