Studies On Process Of Producting Pectinase From Apple Pomace And Cottonseed Meal By Aspergillus Niger HG-1 In Solid State Fermentation

Pectinase is one of the fourth of the Enzyme Preparations in the world and is collective call for any enzyme which breaks down pectic substance and pectinase is widely used in Foods, Medicine, Environmental Protection, Textile, Brewing, Detergent field.Primarily, it sontains components such as polygalacturonase, pectinesterase and pectinlyase. Each component differs from others in the mechanism of reaction and its characteristics.Aspergillus niger was the major genera of microorganisms producing the pectinase in industry. In order to exploit the capacity of Aspergillus niger in producing the enzyme, it is studying on process of producting pectinase from apple pomace and cottonseed meal by Aspergillus niger HG-1 in solid state fermentation in this paper.It is on optimizing the culture medium and fermentation conditions of Aspergillus niger HG-1 to produce pectinase from apple pomace and cottonseed meal in solid state fermentation, and to reveal some prime characteristic of the pectinase in this paper. In this study, Monofactorial and orthogonal experiments were adopted in solid state fermentation. The results show that the activity of pectinase reached 22248 U/g when Aspergillus niger HG-1 was cultured at 30℃for 48h in the optimized medium containing 10g apple pomace,10g cottonseed meal,0.2g (NH4)2SO4 and 0.06g KH2PO4 at the optimized conditions including moisture content of solid substrate 60% (w/w) and 20g dry substrate /250ml flask.The optimal temperature and pH of pectinase reaction were at 45℃and pH5.0. Pectinase was stable below 50℃and between pH3.0 and pH6.0. Ca2+,Mg2+ and Fe2+ increased pectinase activity whereas Ba2+,Mn2+,Zn2+ obviously inhibited it.The results showed that it was technically and economically feasible to produce pectinase from apple pomace by Aspergillus niger HG-1 in solid statefermentation. It was also an effective way to make the best of apple pomace,an agricultural waste produced in larger quantities in China.