| In this study, a kind of nutrition bone meal microencapsulates from yak's bone was developed with technology of high-pressure steam sterilization, superfine comminution and spray-drying. The feasibility of spray drying for making use of a certain proportion of yak's bone, CCP and inulin as microcapsule core material, and added wall material for the comprising the entirety of microcapsule, quality analysis for microencapsulation nutrition bone meal were made as well as. The main results were as follows:Results of yak bone meal:The optimum combination of bone size parameters were ascertained by using of L9(34) orthogonal test, primary granularity 5cm, high-pressure time 150min, grinding gap 5μm and 3 times for glue milling. The minimal granularity of bone paste was 12.1μm, and was for the semi-transparent state.Results of spray-drying parameters make using of yak bone powder: CCP: inulin as mixed-core material in the proportion of 70%: 5%: 25% were as flowing:Results of wall material choosing: gelatin + sucrose, gelatin +β-cyclodextrin and soybean isolate protein+β-cyclodextrin were chose as three kinds of different wall material respectively, the optimal parameters and film-forming rate of three kinds of wall material were determined by single-factor test screening, U6(66) uniform design and bivariate polynomial stepwise regression analysis.By comparative researches of final results from three kinds of wall material, gelatin + sucrose were the most suitable wall material and the best ratio of gelatin to sugar was 1:6, the film-forming rate was 84.3%.The appropriate ratio of core material to wall material was 25:75; the ratio of solids (core-wall material) to water was 50:125.The optimal parameters for spray-drying were: inlet air temperature 200℃, outlet air temperature of 80℃, homogeneous pressure 40Mpa, homogenization temperature 60℃. Correlation analysis showed that there were significant correlation between the core-wall ratio and the film-forming rate, and negative correlation between the square of core-wall ratio and film-forming rate, but no significant. Homogeneous pressure and temperature, as well as the inlet air temperature and the outlet air temperature were positively correlated to and significant impacted on the film-forming rate.Results of electron microscopy for microencapsulation were: grain size 6~25um, the average grain size 16μm, wall thickness 3~5μm. Products were a bit of specific to the taste and smell of animal protein, as ivory white powder, no-bonding phenomenon, uniform in particle size, no sag on particle surface.The proportion of Yak bone meal:Physico-chemical indexes were: Static angle of 28~32°, moisture content 6.96 g/100g, protein 21.7 g/100g, total sugar 28.2g/100g, Ca 12935.9mg/100g,P 5257.1mg/100g. As 0.07mg/kg,Pb 0.21mg/kg,Hg 0.03mg/kg,total bacteria≤30000cfu/g. Microbial indexes were: coliforms≤90MPN/100g, fungus≤50cfu/g, non-pathogenic bacteria were detected out. Meeting the qualification of enterprise standards Q/LQS 001-2007. |
PDF Full Text Request