Studies On The Separation/Enrichment Of The Active Constituents In Traditional Chinese Medicine And Macrolide Antibiotic In The Environment By Solvent Sublation

Traditional chinese medicine is very important for medical care system and prophylactic of the people all along,so to separate and enrich the active constituents of traditional chinese medicine is a concernful research,and how to extract and separat efficently and low-pollution is the problem.Macrolide antibiotics such as roxithromycin,acetyl spiramycin, tilmicosin,are widely used in clinical treatment and aquaculture industry, residues in the environment will harm humans and the environment.And antibiotic residues in the environment is the amount of trace level,it is important for us to set up an effective separation and enrichment methods for monitoring antibiotic residues in the environment.The aim of the project was to develop a fast and reliable method for the separation/enrichment of the active constituents in traditional chinese medicine and macrolide antibiotic in environment samples.The method was based on aqueous two phase-solvent sublation combined with spectrophotometric spectrometry or high performance liquid chromatography.The method takes a new direction for the flotation process with vast development foreground.The main research contents and conclusion are as follows: 1.The behavior of separation/enrichment on the the active constituents of alpiniae officinarum was studied by GSS.The effects on the sublation efficiency was investigated and the optimal condition of the solvent sublation was obtained:the sublation solvent was n-butanol,the pH was 1.25,NaCl added was 5 g,N₂ flow rate was 30 ml/min,the sublation time was 50 min.The flotation efficiency under the optimum conditions was 77.32%with enrichment multiple 7.78.And compared with the solvent extration,the solvent sublation was better.2.The behavior of separation/enrichment on the the active constituents of cortex fraxini was studied by GSS.The effects on the sublation efficiency was investigated and the optimal condition of the solvent sublation was obtained:The sample concentration is 9.50μg/ml, the sublation solvent was n-butanol,the pH was 3,NaCl added was 5 g, N₂ flow rate was 25 ml/min,the sublation time was 40 min.The flotation efficiency under the optimum conditions was 52.94%with enrichment multiple was 3.9.3.The separation/enrichment of roxithromycin was studied based on the system of aqueous two phase-gas solvent subblation,and the method of sulfuric acid color was established to determin roxithromycin enriched. The optimal condition of the solvent sublation was obtained:the sublation solvent was isopropanol,the salting-out agent was K₂HPO₄ with 48%(g/100ml),the pH was 9,N₂ flow rate was 15 ml/min,the sublation time was 30 min.The flotation efficiency under the optimum conditions was 93%,enrichment multiple was 9.3.And compared with the solvent extration,the solvent sublation was better.4.The separation/enrichment of tilmicosin was studied based on the system of aqueous two phase-gas solvent subblation,the method of HPLC was established to determin tilmicosin enriched.The optimal condition of the solvent sublation was obtained:the sublation solvent was n-propanol,the salting-out agent was(NH₄)₂SO₄ with 36%(g/100ml),the pH was 3,N₂ flow rate was 20 ml/min,the sublation time was 30 min.The flotation efficiency under the optimum conditions was 84%,enrichment multiple was 16.8.And compared with the solvent extration,the solvent sublation had many merits,such as high enrichment factor,large volume of water sample,n-propanol with less,no emulsification,etc.The method is suitable for determining the antibiotics residue in environment sample.