Study On Effect Of Petroleum Hydrocarbon Pollution To Activity Of Enzyme Of Hemicentrotus Pulcherrimus And SNP Analysis Of Genes

Oil pollution is one of the main pollution in the marine environment. In the coastal marine environment, petroleum hydrocarbons are liable to deposition and and accumulation, which leads to a higher level of oil contamination in the seabed. In this paper, model organisms-sea urchin, Hemicentrotus Pulcherrimus was selected as test organism, to study the acute toxicity effects of petroleum hydrocarbon pollution on the antioxidant defense system and SPLOX, WNT8 of the embryos of sea urchins from molecular level to explore the oil pollution toxicity mechanism on aquatic life and to find suitable biomarkers for oil pollution detection.The effects of activity of biological antioxidant enzymes system to is an important means of molecular toxicology to assess biological toxicity. The effects of No.0 WAFs diesel on the antioxidant defense system in Hemicentrotus Pulcherrimus after low-dose exposure were investigated. The results show the dose-effect and time-effect relations were between the biochemical response and petroleum hydrocarbon pollution. The time-effect pattern of antioxidant defense system follows the law of increasing first and then decreasing.SPLOX is an important gene that control development of gut of sea urchin and WNT8 is to control nervous system. From the molecular level, complement SPLOX and WNT8 gene mutation was detected through PCR-SSCP technique and sequence determination. Analysis of test results found that in SPLOX, 1 primers (primers G-1) of the PCR product exist the polymorphism, single base mutation have taken place in coding region of the 41, with the conversion of A→G, where amino acid changed from lysine into arginine. And WNT8 gene mutation detection results show that 1 primers (primers M-2) of the PCR product exist the polymorphism, single base mutation have taken place in coding region of the 706, with the conversion of T→C, where amino acid changed from phenylalanine into leucine. The results show that petroleum hydrocarbon pollution could cause DNA mutations in sea urchin, and show some genetic toxicity.