Study On Toosendanin Extraction Form Chinaberry Barks And Separation Of Toosendanin Extraction Solution

For Anhui real estate natural plant Neem trees, Soxhlet - ultrasound method from the Neem tree bark extract active ingredients Toosendanin, multi-level film - Macroporous combined process of the extract were isolated, and to study the active ingredients from associated with the separation mechanism and theory.Soxhlet - ultrasound combined method to extract from the bark of Melia azedarach L. Optimized conditions were: extraction solvent 70% ethanol/water solution, solid to liquid ratio of 1:15 g.mL-1, reflux cycle 11 min, extraction time 3 h, ultrasonic power 100 W, ultrasonic frequency 40 kHz ,under the conditions of extraction rate was 1.789 %.After the extract concentrated by distillation to 50 mL n-butanol extraction of 24 h, extraction rate was 98.12%.Respectively 0.45μm-20 ~ 50 KDa-10 KDa multi-stage filtration - ultrafiltration membrane combined process and multi-level - combined technology macroporous extract Toosendanin study were isolated. Combined in a multi-stage membrane process, the optimal operational temperature of 45℃, pressure of 0.10 MPa, pH value 7.0 solution under the conditions of the transfer Toosendanin was 72.50%, impurity removal rate of 95.69%, purity Toosendanin income increased 18.75%. In the multi-level film - combination of macroporous resin adsorption process, focusing on the static adsorption and dynamic adsorption and desorption processes, optimization of S-8 macroporous resin combined process and the parameters were studied in the optimization of the adsorption - wash off conditions, the purity Toosendanin from 0.96% to 86.2%.Separation of micro-membrane extraction Toosendanin object of study, the resistance of microfiltration based on the established part of the block model; Optimal operating conditions of experimental results verify the accuracy of the model to determine the scope of application of the model is as follows: pressure 0.06 ~ 0.10 MPa, temperature 25 ~ 55℃, pH value of 3.0 to 7.0.The process and kinetic model of toosendanin extraction form Chinaberry barks using solution extraction method was studied, and make sure the primary parameter in the equation testify the reliability of the kinetic model.Found that, within the diffusion process is Toosendanin extraction process control steps, within the spread includes the following two processes: The result showed internal diffusion process is the control steps of the toosendanin extraction process, moreover, extraction process can divided into two processes, rapid diffusion process -the toosendanin in broken cell and cell interstice diffusion to solvent rapidly because of small resistance, slow diffusion process- the toosendanin in whole cell diffusion to solvent slowly. so improved the kinetic model, the revised model have good coincidence with the experimental results.