Study On The Characteristics Of Photosynthetic Bacteria Colony In Continuous Hydrogen Production

This paper is based on research supported by "863" project(No. 2006AA05Z119) and the National Natural Science Foundation (No.50676029). This paper studies the characteristics of photosynthetic bacteria colony used glucose and pig manure wastewater in continuous hydrogen production process, it provides a scientific reference for further improving photosynthetic bacteria continuous hydrogen production technology. The result showed that:(1) It observed patterns of photosynthetic bacteria under the microscope, we found mixed bacteria are mainly spherical, long rods, short rods, spiral, arc, etc. Some cells are single growth; some cells also assembled into a variety of cells to form filaments or filamentous chains. Many photosynthetic bacteria are seen in the movement from the suspension, this is because most of them have long flagella.(2) Through determination of absorption spectra of living cells used UV scanning, photosynthetic bacteria were 230nm, 250nm, 806nm, 860nm department has absorption peaks.(3) No matter what kind of hydrogen production using matrix, when the reactor started operation, the greater photosynthetic bacteria inoculums, the more the number of photosynthetic bacterium, especially in the first day of the proliferation of more photosynthetic bacteria. As an added photosynthetic bacteria for hydrogen production, the steady decline in photosynthetic bacteria can not meet the demand for continuous hydrogen production; every 8 days plus a photosynthetic bacteria, photosynthetic bacteria just to meet the requirements of continuous hydrogen production. Photosynthetic bacteria proliferation only in the first days, when the next day to begin mass production of hydrogen photosynthetic bacteria, the photosynthetic rate on the proliferation of bacteria is much less than the speed of his decline.(4) directly affect the concentration of photosynthetic bacteria photosynthetic bacterial hydrogen production, the greater the concentration of photosynthetic bacteria, hydrogen production is also more.(5) The photosynthetic bacteria in 2 #, 3 # the maximum concentration of photosynthetic bacteria, the largest amount of hydrogen. Followed by 1 #, 4 #, at least. Hydrogen production in photosynthetic bacteria growth period of stability, 2 #, 3 #, the largest compartment on hydrogen production, indicated that the two compartments in the growth of photosynthetic bacteria most stable phase, the fourth compartment of the photosynthetic bacteria most of the recession period. The 1 # compartment, especially the first day of photosynthetic bacteria in the proliferative phase, almost no gas.(6) the photosynthetic bacteria use glucose, respectively (where appropriate by adding a variety of trace elements, glucose does not produce light hydrogen) to hydrogen medium, the use of pig manure wastewater pretreatment (with 1% glucose to do hydrogen primer) substrate for the production of hydrogen, the concentration of each compartment comparison of photosynthetic bacteria. Photosynthetic bacteria found in mixed flora can make better use of pig waste in small molecule organic compounds, which may be due to the components of the glucose solution in a single, pig waste water rich in nutrients, and mutual assistance between the mixed flora can make better use of manure nutrients in wastewater.(7) the use of pig manure wastewater to produce hydrogen, the continuous hydrogen production reactor compartment of the PH value of the various changes in the 7-6.5, between fits of photosynthetic bacteria growth and reproduction and hydrogen requirements. Hydrogen production using glucose, the reaction mixture more acidic. PH refers to the use of pig manure wastewater is relatively stable.