| Dynamic high pressure microfluidization (DHPM) treated on soybean soluble polysaccharides (SSPS) composition, structure and functional properties, is good for understanding the molecular structure in the role of the polysaccharide to DHPM mechanism, and comprehending SSPS diversifications in relationships between structure and function.1,The soybean purification experimental results showed:Taiwan 75-1 soybean (28.2% sugar content) was selected to be our experimental subjects. SSPS was extracted by high temperature-polar pH value method (compared to enzymatic method).The main points of extraction:alkali deproteinized procedure:liquid ratio 1:10, pH value 9.5; acid extracting SSPS procedure:temperature 120℃, pH4.0.The total yield is 41.83 percent. Ion exchange resin chromatography was used to purify polysaccharide, determining the sample volume was 50mg, buffer pH value is 6.8. Classification of the SSPS obtained two components of SSPS-1, SSPS-2 by detecting the molecular weight spectra and UV spectra, SSPS-2 molecular weight is 91.5kD, protein content is 86.7%,which was the high-binding protein content in single polysaccharide. SSPS-1 was conducted as the object of DHPM treatment.2,SSPS-1 contains five single peak, and the relative molecular weights are 733kD,295 kD, 25 kD,6223D and 1589D respectively, which was considered to be the complex polysaccharides containing a small amount of protein.lt was determined to be theβ-pyranose, and the main chain involved of rhamnose and galacturonic acid, which was connected by 1-3-Rha, and 1-4-GalA.Other components of the polysaccharides were arabinose, galactose, wood glucose and mannose through the GC, infrared scanning and methylation analysis.3,The analysis of the components from SSPS-1:dealed with DHPM processing, large molecular weight components of SSPS-1 decreased,and small molecular weight components lowed, with middle molecular weight fractions increased.The mode of DHPM process on SSPS-1: macromolecules intended to lower molecular weight while low molecular weight converted to high molecular weight aggregates. SSPS-1 changed into a spherical enlarged end of the "match stick"-like structure of ordered arrangement from the needle-like stacking arrangement.The main chain monosaccharides content of SSPS-1 treated by DHPM:Rha and GalA,dropped down and part of the single-sugar side chain such as Ara, Gal, Fuc and Man reduced, and accompanily Xyl, Glc, GlcA was not detected. The content of Glycosidic bonds connecting the main chain of (1→3) Rha and (1→4) GalA decreased, while the content of glycosidic bonds connecting the main branch chain increased. Generally DHPM processing will increase the uronic acid content, but the polysaccharides ring configuration has not changed.4,The physical and chemical Properties of SSPS-1 treated by DHPM:SSPS-1 treated by DHPM with the increase of dealing times,its size distribution, Zeta potential, apparent viscosity, were decreased first, then showed a small peak. The emulsification, emulsion stability of SSPS-1 treated by DHPM processing at 150MPa,4 times showed the maximum value. SSPS-1 treated by six times, reached the highest position of foaming capacity while foaming stability treated by 2 times increased to the peak.5,The physiological function of SSPS-1 treated by DHPM:with DHPM treatment on the SSPS-1, the bifidobacterium flora and lactic acid bacteria have increased, followed by the number of E. coli decreased in the fecal flora and intestinal bacteria of the healthy mouse. In another way,SSPS-1 increased the beneficial bacteria (bifidobacterium bacteria, lactic acid bacteria) in a multiplier effect, and decreased the harmful bacteria (E. coli) an inhibitory effects. The two beneficial bacteria increased ratio effected by SSPS-1 is higher than the percentage inhibition of harmful bacteria. SSPS-1 treated by DHPM processing at 150MPa,4 times, have an effect on beneficial bacteria with the highest proportion and the inhibition of E. coli with the highest proportion.The absorption of bile acid on SSPS-1, was a process of adsorption analysis. The treatment of DHPM to SSPS-1 on bile acid adsorption added to the amount of absorption, simultaneously extended the release time of bile acids. The adsorption of SSPS-1 on Ca2+, Mg2+ is a dynamic equilibrium process, whlie the process on Cu2+, Pb2+ adsorption is a relatively stable, slow-released phenomenon.In summary:the treatment of DHPM can make the higher and lower molecular weight of classified SSPS(SSPS-1) moved closer to the medium molecular weight.Further the SSPS-1 changed in the glycosidic bond ratio, the monosaccharide composition and the functional groups, resulting the diversification of its functional properties and physiological properties (the size distribution, the apparent viscosity,the foaming capacity and foaming stability, emulsifying capacity and emulsifying stability,the fecal flora and intestinal bacteria from a healthy mice, the bile acids and Ca2+, Mg2+, Cu2+, Pb2+ metal ions adsorption capacity).
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