Analysis Method Of Nsaids Drugs And Interaction Between Drugs And Biomacromolecule By Spectrometry

The rapid development of analytical chemistry makes it break through of a single subject area, and accomplish the transition from analytical chemistry to the analytical science covering many subjects such as chemistry and mathematics, physics, computer science, biology, and developing as a multidisciplinary subject. One of the directions of development of analytical chemistry is to improve the sensitivity and selectivity of analysis, the anothers is to expand vigorously its space of development to life sciences field.Analysis determination method of nsaids drugs and the interaction of nsaids dr-ugs with biomacromolecule were studied by photometry and fluorescence spectrosco-py method in this paper. cresol red as colour reagents was presented first time,we est-ablish a method for determinstion of nsaids drugs by spectrometry, which processing high sensitinity and good selectivity. Study on the interaction mechanism between ns- aids drugs and biomacromolecule of original investigation meanwhile. The Stern-Vol-mer quenching constant Ksv,bimolecular dynamic quenching rate constant Kq,co-nstant K,binding site number n,thermodynamic parameters and force type,constant r and structure change of drugs to biomacromolecule were obtained.The paper consis-ts of two aspects as followsPart 1 Fading spectrophotometric method for the determination of nsaids drugs with dyeThe interaction of nsaids drugs (piroxicam,fenbufen,benzbromarone) with dye(cresol red)was studied by photometry method. Founding a new fading spectrophotometric determination method of dye reacted with nsaids drugs to form an ion association complex, which caused he fading of the dye solution. Proposed analytic methods are simplicity convenience, high sensitinity, wide linear range, good selectivity and studying the optimum conditions of systems.at the meantime, which were applied satisfactority to the determination of medicinal preparation and practical samples with the results were satisfying.Part 2 Studied the interaction of nsaids drugs and biomacromolecule by spectrometry.The interaction between nsaids drugs (lornoxicam,fenbufen) and bovine serum albumin(BSA) was studied with fluorescence spectra and UV-visible absorption spectra in the presence of simulating physiological systems.It showed that the complex formation of BSA and drugs resulted in the quenching of·the intrinsic fluorescence of BSA, and the quenching was a static quenching procedure. The Stern-Volmer quenching constant Ksv,bimolecular dynamic quenching rate constant Kq,binding site number n among apparent binding constant KA were measured according to sternvolmer equation and static quenching formula.The same to the distance between BSA and drugs with the Forster nonradiative energy transfer theory.On the basis of the thermodynamic parameters, we confirmed that the main sorts of binding force between drugs and BSA is electrostatic force. Meanwhile, synchronous fluorescence and threedimensional fluorescence spectra were used to investigate the structure change of BSA with the addition of drugs. Furthermore, the effect of coexistence metal ions on the binding constants of drugs with BSA was also discussed.