Study On Macrophage Migration Inhibitory Factor - 173G / C Gene Polymorphism And Susceptibility To Primary Gout In Chinese Han Population

Objective:To explore gene polymorphism of-173G/C (rs755622) in the promoter of macrophage migration inhibitory factor (MIF) gene in a Chinese Han male population, and to investigate the relationship between gene polymorphism of MIF and the susceptibility to gout.Methods:Gout patients and healthy controls were enrolled.The possible association of the polymorphism of MIF173G/C and gout in Chinese were investigated and genotype frequencies and allelic frequencies were analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP) method. We used Hardy-Weinberg to confirm the representativeness of the samples. X2test was used to performed the comparisons between the groups. We performed the genotype-phenotype correlation analysis by ANOVA.Results:The patients of gout group have a high levels of uric acid, TG, TC, blood glucose, creatinine and urea nitrogen (P<0.01). The frequencies of GG. GC、CC genotypes were60.64%(208cases),35.86%(123cases) and3.50%(12cases), respectively among gout patients, while they were66.5%(252cases).29.8%(113cases) and3.7%(14cases), respectively among the controls. There was no significant difference in MIF-173G/C genotype frequencies between the gout patients and the healthy controls.(P=0.223). The allele frequency of G、C in gout patients were78.57%(539cases) and21.43%(147cases), while the healthy controls were81.4%(617cases) and18.6%(141cases).No significant difference was found between the two groups. We divided the gout patients into tophi patients and non-tophi patients. Both the allele frequencies and the genotype distributions did not give evidence of the significant associations between them (P=0.117,P=0.510). The ANVON shows that no remarkable differences in phenotype among gout patients of different genotypes (P>0.05).Conclusion:The genotype of-173G/C in the promoter of MIF gene is not associated with gout in male population.