| Objective: Analysis of single nucleotide polymorphisms in 10 locations of TLR4 gene in gout patients with hyperuricemia in Han nationality in Xinjiang.Methods: A total of 1209 cases were selected from the First Affiliated Hospital of Xinjiang Medical University and the Chinese Medicine Hospital of the Autonomous Region in 2015-2018,including 317 cases of gout,385 cases of hyperuricemia,and 507 cases of healthy physical examination in the same period.All subjects signed informed consent.The book is included and excluded according to the gout diagnostic criteria.All subjects collected general data and peripheral venous blood samples,gout patients and hyperuricemia patients collected clinical data,and all subjects were examined in the laboratory to detect biochemical indicators and genotyping of 10 sites of TLR4 gene.The data was entered using Excel software and SPSS22.0 software was used for data analysis.Results: The onset age of the gout group was(46.96±12.48)years,the course of disease was(6.64±7.25)years,among them,there are 33 cases had tophi(10.41%),and 18 cases had kidney stones(5.7%).The first attack involved 57.65% of the first metacarpophalangeal joint,the ankle joint was 17.35% and the knee joint was 8.67%;Obesity,hypertension,hyperglycemia,hypertriglyceridemia and kidney injury were risk factors for gout and hyperuricemia(P<0.05,OR>1),while regular exercise was a protective factor(P<0.05,OR<1);10 SNPs of TLR4 gene met HWE balance test in the Han population in the region(P>0.05);the was no statistical correlation between the 10 locus gene mutations and the incidence of gout and hyperuricemia(95%CI spans 1);there was no statistically significant association between TLR4 gene rs10759932,rs1554973,rs1927906,rs2149356 and rs2737190 polymorphisms with gout and hyperuricemia in the dominant,recessive,and codominant modelsthere was no statistically significant association between the two groups(95%CI spanned 1);Ccr in rs2149356 locus in gout group had statistically significant differences in GG,GT and TT genotypes and rs2737190 Ccr in AA,AG and GG genotypes in the gout group all were statistically significant(P<0.05).There was significant difference HDL in GG,GT and TT genotyping between rs2149356 locus in hyperuricemia group(P<0.05).The general indicators of the same genotype at 8 loci were significant different between the gout group,the hyperuricemia group and control group(P<0.05).;The risk of hyperglycemia in the gout group of rs1554973 and rs1927906 was significantly different from that of the control group(P<0.05).The risk of obesity in the gout group of rs4986790 and rs4986791 was statistically different from that of the control group.The difference was statistically significant(P < 0.05);the risk of hypertension in the hyperuricemia group of rs1554973 and rs2770150 sites was statistically significant(P<0.05),the risk of hypercholesterolemia in hyperuricemia group of rs2149356,rs2737190,rs2737191 and rs7873784 was statistically different from that of the control group(P<0.05).The risk of obesity induced by hyperuricemia in the rs4986790 and rs4986791 sites was statistically different from that of the control group.The significance of learning(P<0.05);the GTT haplotype consisting of rs2737190,rs10759932 and rs2149356 polymorphisms was the protective factor of gout(OR=0.689,P<0.05);the interactions between the TLR4 genes was no statistically significant(P>0.05).Conclution: The onset of hyperuricemia and gout is closely related to the components of the metabolic syndrome.Alcohol,obesity,hypertension,hyperglycemia,hypertriglyceridemia,and kidney damage all increase the risk of hyperuricemia and gout.The 10 polymorphisms of TLR4 gene were not associated with the occurrence of gout and hyperuricemia.The GTT haplotype composed of rs2737190-rs10759932-rs2149356 was a protective factor for gout. |
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