Effects Of Safflower Sorghum Gynostemma Decoction On Blood Lipid And Hemorheology In Hyperlipidemic Rats

Objectives:To explore the lipid-regulating effect and mechanism of (Hawthorn Gynostemma Tang, HGT) on hyperlipidemic rats, we observed blood lipid level, blood rheology changes and other aspects of hyperlipidemic rats model fed with high fat diet.Methods:The Wistar rats (n=60) were divided into normal control group (n=10) and hyperlipidemic group (n=50). Hyperlipidemia model rats were divided into hyperlipidemia model group, low dose HGT group (5.3g·kg·d-1), medium dose HGT group (10.6g·kg·d-1)and high dose HGT group(21.2g·kg·d-1) and the positive control group (Xuezhikang treatment). Normal control group was fed with basal diet, and the other groups were fed with hyperlipidemic diet. Using the method of intragastric administration, the normal control group and hyperlipidemic model group were administrated with the same volume of saline. Indicators were detected after7weeks:1. Changes of weight, food intake and fecal fat content in rats during the experiment;2. Serum lipid levels(TC、TG、LDL-C、HDL-C);3. Hemorheology (whole blood viscosity, plasma viscosity, red blood cell aggregation index, red cell deformability index, hematocrit, etc.);4. Rat liver, spleen and heart were isolated for weighing and calculating organ index; detecting liver cholesterol; producing liver histologic section, observing tissue pathological changes under the microscope.Results:1. Rat hyperlipidemia model could be built by hyperlipidemic diet feeding for7weeks. Weight gain of rats could be inhibited through treating with HGT for4weeks, and the food intake inhibition in a dose and time dependent correlation manner were also observed. Fat content in rat feces were detected and compared to the model group, increased fat content were found in the HGT group significantly(P<0.05), indicating that HGT can increase fat excretion in feces and inhibit intestinal fat absorption.2. Compared to the hyperlipidemia model group, serum TC, TG and LDL-C levels were all decreased in the HGT group. Especially, TC and TG levels reduced extremely significantly(P<0.01), while LDL-C levels reduced significantly(P<0.05). TG/HDL-C, LDL-C/HDL-C ratios and atherosclerosis index (AI) were also decreased significantly(P<0.01), indicating that HGT had certain lipid-regulating effect on hyperlipidemic rat model.3. HGT can decrease the hemorheology index (whole blood viscosity, plasma viscosity, red blood cell aggregation index, red cell deformability index, hematocrit), improve blood rheology obstacles on hyperlipidemic rats, increase the tissues and organs blood supply, and improve the symptoms of cardiovascular and cerebrovascular diseases.4. Compared to the hyperlipidemic model group, low dose HGT, medium dose HGT and high dose HGT can effectively reduce liver cholesterol levels(P<0.01). Also, morphology observation showed that HGT could improve the liver lipid infiltration in a certain extent.Conclusions:The monarch drug hawthorn in the Medicative Diet was in favor of strengthening spleen and stomach, eliminating food overload and fat. The ministerial drugs pseudo-ginseng and gynostemma were effective in accelerating blood circulation and eliminating expectorant. Adjuvant drugs coix seed, caulis perllae, together with conductant drug raphani could strengthen spleen-stomach, subdue the adverse flow of Qi and eliminate dampness. Six drugs combined formulations can improve the function of the spleen and stomach, eliminate phlegm and dampness, clarify, reduce stasis and smooth meridians, guide the Qi, thus were effectively in decreasing blood lipid levels. Medicative Diet HGT have significantly improved the weight gain, abnormal lipid levels, blood rheology changes and so on in hyperlipidemia rat model.