| Lipometabolism disorder is one of clinic familiar disease. In recent years, It is realized that dyslipidemia plays an important role in the generation and development of coronary heart disease (CHD) and atherosclerosis(AS). Danshen and shanzha are traditional drug to treat lipometabolism disorder. In the studies, the compounds of lipid-regulating function were extracted, isolated and determined in the danshen and shanzha. The best proportion of lipid-regulating extraction of danshen and shanzha were elected with hyperlipidemic mice. Their lipid-regulating effects were observed in hyperlipidemic rats and rabbits. On this basis, cell and molecular mechanisms of lipid-regulating of the best proportion were studied.1.Extraction, isolation and determination of lipid-regulating function part in danshen and shanzha(1). Study on extraction process which effective part of lipid regulating of danshenAccording to properties of total-phenolic acid of danshen selected water was as extractive solvent. The main influence factors including extractive time, extractive times and solvent content were defined by orthogonal design. To find out the optimum extraction process for total-phenolic acid of danshen, in the extractive fluid tannin and other large molecular substance were deposited by gelatin and alcohol. Total phenolic acids of danshen were extracted by ethyl acetate on the basis of regulating propriety pH of fluid.The results showed that the extraction process was simply, good stability and was available for industrial prodution.(2). Study on extraction process which effective part of lipid regulating of shanzhaAccording to properties of total triterpenic acid of shanzha, selected alcohol was as extractive solvent. The main influence factors including extractive time, extractive times, solvent content and alcohol concentration were defined by orthogonal design. To find out the optimum extraction process for total triterpenicacid of shanzha, in alcohol extractive substance, substance which soluble in water was washed by water and extracted by ethyl acetate.The results showed that the extraction process was simply, good stability and was available for industrial production.(3) Determination of danshen,shanzha and their effective part of lipid-regulatingThe effective part of lipid regulating of danshen is total-phenolic acid. Using active compound danshensu,protocatechualdehyde are index of this part. To determine the contents of effective compound and extractive substance HPLC and colorimetric method.were used. The results showed that the content of danshensu was best in danshen of henna louyang. It was 0.477%. The content of danshensu of effective part was 12%. The average recovery of danshen was 98.75%. RSD was 0.3%. The average recovery of extractive substance was 99.81%. RSD was 0.47%. The content of total-phenolic acid in extractive substance was 80%, which detected by colorimetric method. The average recovery was 99.77%. RSD was 0.31%.The effective part of lipid regulating of shanzha is total- triterpenic acid. Using active compound ursolic acid in this part as an index. Adopting HPLC and colorimetric method to determine the contents of effective compound and extractive substance. The results showed that content of ursolic acid in shanzha of shangdong qingzhou, was best. It was 0.454%. The content of ursolic acid of effective part was 55%. The average recovery of shanzha was 98.63%. RSD was 0.48%. The average recovery of extractive substance was 99.29%. RSD was 0.48%. The content of total triterpenic acid of extractive substance was 60% by colorimetric method. The average recovery was 97.69%. RSD was 1.07%.The results showed that the method was simple, reliable, and accurate and could be applied to the quality control of the preparation.(4) Isolation and determination of active constituents in effective parts.15 compounds were isolated from the effective part of lipid regulating of shanzha. On the basis of the properties and spectral analysis, their structures...
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