Study On The Immunoregulatory Mechanism Of Cang'ai Volatile Oil On Lung Qi Deficiency Model Rats

Objective:The volatile oil of Cang Ai (VOCA) was come from Prof. XiongLei clinical experience.It was made of Artemisia argyi,Agastache,etc.Clinical studies had demonstrated that the VOCA had obvioustherapeutic effects on the Lung-Qi Deficiency Syndrome.Previousstudies have shown that the volatile oil had inhibitory effect on thecommon strains of AURI,such as staphylococcus aureus,Group Astreptococcus pneumoniae and Group B Streptococcus.VOCA could alsoimprove the mucosal immune function in mice.Based on the aboveresearch,we planned to duplicated the rats model of olfactory bulbdamage to make clear the VOCA could regulate the body’s immune byintranasal brain targeting pathway.in order to further clarify thatintranasal medication of VOCA was scientificity and rationalitymeanwhile,to provide the experimental basis for clinical medication.Method：The40SD rats were randomly divided into five groups accordingto the weight,including blank control group,model group of Lung-QiDeficiency(model group A),VOCA treatment group of Lung-QiDeficiency(model group A+VOCA),model group of damagingolfactory bulb and Lung-Qi Deficiency(model group B),VOCAtreatment group of damaging olfactory bulb and Lung-Qi Deficiency(model group B+VOCA),every group had8rats.The ratsmodelsof olfactory bulb damage and Lung-Qi Deficiency wereduplicated,while giving the VOCA to rats.After30daysadministration,the serum IL-6,IL-1β,IFN-γ levels were tested byELISA.Functional Magnetic Resonance Imaging was used to showfunctional activity in brain regions relating to nasal administration,thenwe detecte GFAP and OX42which are specific marker of astrocytes andmicroglia by fluorescent immunohistochemical method.Result：1. Compared with the blank control group,levels of IL-6in model groups A and B were increased(P＜0.05).Compared with themodel group,the levels of IL-6in treatment groups of the VOCAwere decreased,the difference was statistically significant (P<0.01);However,there was no statistically significant difference between model group A+VOCA and model group B+VOCA(P>0.05).2. Compared with the blank control group,level of IFN-γ in modelgroup B was decreased (P<0.05);which in model group A was decreasedbut there was no statistically significant difference(P>0.05);Comparedwith model group B,level of IFN-γ in model group B+VOCA wasincreased obviously (P<0.05);Compared with model group A,level ofIFN-γ in model group A+VOCA was increased,but there was nostatistically significant difference(P>0.05).Compared between drug groups,there was no statistically significant difference (P>0.05).3. Compared with the blank control group,levels of IL-1β in modelgroups A was increased(P<0.01);Compared with model group A,level ofIL-1β in model group A+VOCA was increasedobviously(P<0.01);Compared with model group B,level of IL-1β inmodel group B+VOCA was no significant change（P>0.05）,Comparedbetween drug groups, there was no statistically significant difference(P>0.05).4.The Blank control group and model rat of olfactory bulb damageand Lung-Qi Deficiency were Scanned by fMRI after10min,25min,45min,70min,100min by intranasal administration of thevolatile oil of Cang Ai.The fMRI displayed that static level of brainfunction of two groups were increased and distribution area wereexpanded significantly.After administration10min,high activityareas(yellow area)were mainly distributed in the sensorimotorarea,cingulated,hippocampus,hypothalamus and olfactory bulb.Afteradministration25and45min,static level of brain function of damagingolfactory bulb with Lung-Qi Deficiency keep a high level in thesensorimotor area,cingulate,hippocampus,hypothalamus and olfactorybulb After administration70min,static level of brain function in basalganglia area of the hypothalamus of damaging olfactory bulb withLung-Qi Deficiency was decreased significantly compared with theprevious point in time.Static level of brain function keep a high levelafter100min,but it was decreased compared with70min. Static level ofbrain function after25and45min,blank control group weredecreased,but after70and100min,static level of brain function returnedto a high level.5. Compared with the blank control group Expression of OX42were enhanced obviously in model group A+VOCA.Conclusions：1. The VOCA could effectively reduce the levels of IL-1β and IL-6effectively in the blood of rats with Lung-Qi Deficiency so to inhibit theinflammatory reaction of rats with Lung-Qi Deficiency;Increasing thelevel of IFN-γ to improve the humoral immune.2. FMRI displayed that static levels of brain function of Multiplebrain regions are increased significantly by intranasal administration ofthe VOCA,which provided preliminary evidence that the drug couldactivate the brain through the olfactory pathway;Immunofluorescenceexperiments of activated brain areas showed that VOCA enhancedexpression of OX42and might activate microglia in brain involving inimmune response.