Study On Quality Evaluation Of Gan Song Herbs

Objective:The genus of Nardostachys DC. includes several species, like Nardostachy jatamansi DC, N chinensis Batalin., N. grandiflora Candolle., etc. Due to these species were excessive types in plant taxonomy, all of these types were merged into one species——Nardostachyjatamansi. Nardostachys jatamansi yields Nardostachys Radix et Rhizoma, which has a long history to be used as a traditional medicine in China, India, Nepal, Ancient Egypt, Ancient Rome, to treat various ailments like abdominal distention, inappetence, vomit and toothache, barbiers. It is also used as an ingredient of cosmetic, soap, aromatherapy and some foods. It’s the typical medicinal plants in Himalayan. In China, it is mainly distributed in southwestern areas, such as Gansu, Qinghai, Sichuang provinces and Tibet. Through reviewing literatures and summarizing the early work of our team, we have found:Usage of N jatamansi in different regions is not identical; Most of Nardostachys jatamansi in producing area and market contains the ground part; In the 2010 edition of China pharmacopoeia, there is only a kind of essential oil in content determination about N jatamansi; There are some problem regarding to the existing HPLC Fingerprint Chromatography, like sample amount is too few to control the quality of medical herbs, there is no study to these two species——Nardostachys jatamansi Dc. and Nardostachy chinensis Batalin.. In order to solve the above problems, this paper have studied:the differences in the quality of N jatamansi from different regions; The differences between the ground part and underground part of’N jatamansi; Improving the quality standard of’N jatamansi inChina pharmacopoeia; Using a large number of samples from a wider range of different regions to comparing HPLCFingerprintChromatography of two species.Methods:Through reading a large number of domestic and foreign literatures about the plants of the genus Nardostachys DC. and summarized. Using the method of combining literature survey and field investigation to investigate the resource distribution and usage state of Nardostachys jatamansi in our country, and collected samples from different regions. The content of the flavonoids and the polysaccharide in N jatamansiwere determined and compared by UV spectrophotometry. The content of the nardosinone was determined by HPLC. The stability of nardosinone and its ethanol solutionwere investigated in different conditions, the results of the study provided the reference for the research of standard substance. Established HPLC Fingerprint Chromatography of Nardostachys jatamansi from different origin.Results:1. Reviewed the quality evaluation ideas and methods of Chinese Materia Medica; Reviewed the resource distribution, species identification, chemical composition and pharmacological effects of the genus of Nardostachys DC.2. Nardostachys jatamansi are mainly distributed in southern Gansu province, south-eastern Qinghai province, Aba and Ganzi area in Sichuan province, and was not found in Yunnan province, its distribution area are decreasing compared with reports in many literatures. It is not used as a medicine plant in some places, and there is no body excavate it. Nardostachys jatamansicontains ground parts mostly. Collected 31 samples of Nardostachys jatamansi Dc. and-Nardostachy chinensis Batalin..3. Established the HPLC determination of nardosinone, improved improving the quality standard of Nardostachys jatamansi n 2015 edition China pharmacopoeia. Determined the content of nardosinone in stems, leaves, flowers and root and rhizome of Nardostachys jatamansi.. The average content is 0.78%,0.0141%,0.0089%,0.0310% in root and rhizome, leaves, stems, flowers respectively.4. The solid nardosinone is stable under the condition of high temperature (60 ℃) and high humidity stability, it is degraded under the condition of light. Nardosinone was completely degradated after placing at pH=3^4 and high temperature. The higher the temperature, the faster degradation rate. Basically stable at alkaline condition and room temperature. Nardosinone was degradated rapidly in artificial gastric juice, basically stable in the artificial intestinal juice. Identifed two degradated products using multiple mass spectrometry information, UV spectral information and the relative retention time, theis molecular weight is 232 and 190 respectively.5. Flavonoids content range of"Nardostachys jatamansi is 1.01%-2.18%, which is 1.5% in average. Polysaccharide content range is 4.68%-14.89%,8.15% in average. There was significant correlation between altitude and total flavonoids, but not much more correlation with total polysaccharides.6. Compared the HPLC Fingerprint Chromatography of two species, processing datas using Traditional Chinese medicine (TCM) fingerprint similarity evaluation system". There is significant differences between Nardostachys jatamansi Dc. and Nardostachy chinensis Batalin., ground parts and underground parts in chemical composition.Conclusion:Through this research, we clarified the condition of resources distribution, usage and some problems. We found the correlation between composition content and altitude. The content of composition of"Nardostachys jatamansi Dc. and Nardostachy chinensis Batalin. is markedly different, we must do further studied to prove whether merger two species into one. It is dignificantly different between ground parts and underground parts in chemical composition, we should not use ground parts as a medicine metiaria. HPLCFingerprintChromatography qualitatively suggests that it is different between these two species.