Detection Of Genetic Variation Of Acer Mono Maxim Mutants Induced By Spaceflight

Acer mono (Acer mono Maxim) is Aceraceae Maple tree the color leaves and an important ornamental plants,By space mutation may have taken place in morphology genetic variation,from the ornamental value for the cultivation of more varieties of Acer mono provide breeding material.In this experiment,the space mutation of Acer mono seedlings cultivated groups and individuals for the materials , Chlorophyll and morphological of the comprehensive clustering. Using RAMP( random amp lified microsatellite polymorphism) markers of their groups genomic DNA were detected, Analysis of space radiation and the control group of seedlings population genetic differences,found whether was occurred by space after Acer mono the genomic DNA genetic variation.On this basis,Individuals from different leaves and roots of extracted genomic DNA,using RAMP(random amp lified microsatellite polymorphism)markers and SSR(simple sequence repeats)markers prove genetic variation within individual,provide a theoretical basis for Acer mono for the space mutation breeding of new varieties of quality. The main results are as follows:Using RAMP(random amplified microsatellite polymorphism)markers 10 pairs of primer combinations on six groups(three treatment and three control)were amplified in 120 individuals with 360 bands,received a total of 256 polymorphic loci polymorphic loci and rate of 71.11%.RAMP marker found High polymorphism within populations,Radiation group more than the control groups .In six population,every population effective number of alleles ranged between 1.4086-1.6485, average 1.5331 ;every population Observed heterozygosity (Ho) values of change in the range of 0.2986-0.3765,averaging 0.3303;every population expected heterozygosity (He) value of the change in the range 0.3454-0.4362,an average of 0.3837,Illustrate the level of polymorphism among populations quite different.By the effective number of alleles detected(Ne)and expected heterozygosity(He)as a standard,the genetic diversity of radiation group sm3 the highest level of genetic diversity were the lowest ck3.Using RAMP( random amp lified microsatellite polymorphism)markers 10 pairs of primer amplified in 120 individuals with 360 bands,Calculated among individuals within a population genetic similarity GS and genetic distance D.In six populations ranged from 0.8724-0.9375,genetic distance between 0.0645-0.1365,Shows a higher degree of similarity and small genetic distance between groups.Space radiation between the three groups and the control of the three groups were relatively high similarity coefficients and genetic distance is relatively small,and space treatment groups and control treatment groups is relatively low similarity coefficients and genetic distance is relatively large.The six populations are clustered into two major clades by UPGMA method based on genetic distance.Space radiation group and the control of the genetic distance of six groups,between 0.0645-0.1365.Populations of three-time repetition of the radiation are clustered into one clade,three-time repetition of the comparison are clustered into the second clade. Display space radiation group and the control groups are differences.Using RAMP markers through 10 pairs of primers were 10 individual leaf and root tissues of 3 repetitions,but each individual was found between the roots and leaves of difference.10 individual leaf and root tissues of radiation 3 times the total amplification of 6326 repeated,in which 399 different bands,the total difference in rate of 6.3%.Application of 10 pairs of SSR primers radiation leaves and roots of individual organizations PCR,its high polymorphism among individuals.10 pairs of primers were 10 individual leaf and root tissues of 3 repeats and found no differences.10 individual leaves and roots of radiation 3 times the total amplification of 1329 repeated,in which 23 different bands,the total difference in rate of 1.7%.In 2009.09 and 2010.09,respectively,in the radiation group and the control of chlorophyll Acer mono seedlings and phenotypic observation of two indicators,based on nine factors Acer mono seedlings,using UPGMA clustering method,clustering results are basically divided into The two groups.The first control group included 2009 and 2010, the control group,the second group of radiation group, including the 2009 and 2010 radiation group,overall,four groups in the two observations,the control and radiation groups to separate groups , indicating that populations Chlorophyll levels and differences in phenotypic traits.