The Critical Factors Affecting Seconary Sex Ratio Of Parasite Wasps Of Brontispa Longissima (Gestro)

When parasitic wasps are breeded in the indoor, the sex ratio is a key factor affecting the whole population, the control of sex ratio is the base of the entire breeding population stability. For fast reproducing a large population of Asecodes hispinarum Boucek and Tetrastichus brontispae and the application of parasitic wasps effectively control the occurrence and hazards of Brontispa longissima (Gestro), we will discuss the key factors affect on the seconary sex ratio of parasitic wasp offspring. Main results are as follows:1. when one parasitic wasp was parasitizing, host density affected significantly fecundity of female Tetrastichus brontispae with nutritional supplement, but not female Asecodes hispinarum. When the female was't supplied the nutrition, the host density have significantly influenced on fecundity of female Asecodes hispinarum, but not female Tetrastichus brontispae. Host density had some effect on the average number of adult per parasitized pupae. The offspring seconary sex ratio obtained added honey and non-honey feeding Tetrastichus brontispae were significantly different, the offspring sex ratio Asecodes hispinarum Boucek were not significantly different. When three pairs of parasitic wasps parasitized multi-host and multi-head of the parasitic wasps parasitized one and three host, the seconary sex ratio of offspring received added honey and non-honey feeding Tetrastichus brontispae has no significant difference.2. In the case of adding honey, the seconary sex ratio of offspring derived from two pairs of Asecodes hispinarum Boucek parasitized multi-head of Brontispa longissima (Gestro) and a number of pairs of Asecodes hispinarum Boucek parasitized two and three heads of Brontispa longissima (Gestro) has a significant influence, but three pairs of Asecodes hispinarum Boucek parasitized multi-head of Brontispa longissima (Gestro) and a number of parasitic wasps parasitized on one Brontispa longissima (Gestro), which offspring sex ratio was no significant difference; the seconary sex ratio of offspring obtained by two pairs of Tetrastichus brontispae parasitized multi-head of Brontispa longissima (Gestro) and multiple pairs of Tetrastichus brontispae parasitized two Brontispa longissima (Gestro) was significant difference, the seconary sex ratio of offspring obtained in many of Tetrastichus brontispae parasitized one Brontispa longissima (Gestro) was no significant difference.3. In the absence of honey circumstances, the seconary sex ratio of offspring obtained three pairs of Asecodes hispinarum Boucek parasitized multi-head of Brontispa longissima (Gestro) and a number of pairs of Asecodes hispinarum Boucek parasitized one and two Brontispa longissima (Gestro) was significant difference, the seconary sex ratio of offspring obtained two pairs of Asecodes hispinarum Boucek parasitized multi-head of Brontispa longissima (Gestro) and a number of pairs of Asecodes hispinarum Boucek parasitized three Brontispa longissima (Gestro) has no significant difference; the seconary sex ratio of offspring obtained by two pairs of Tetrastichus brontispae parasitized multi-head of Brontispa longissima (Gestro) and a number of pairs of Tetrastichus brontispae parasitized two Brontispa longissima (Gestro) has no significant difference.The size of the host hadn't significantly influenced on the reproduction of females of Tetrastichus brontispae and their offspring sex ratio. Unmated females of Tetrastichus brontispae and Asecodes hispinarum Boucek can produce offspring, the number of which offspring had no significant difference in unmated females. Unmated females produce all of the male offspring. Rate of offspring females on mated females of Tetrastichus brontispae was 87.5%, and the rate of offspring females Asecodes hispinarum was 58.59%. There were significant differences among different age of the mating after emergence of Tetrastichus brontispae females on their offspring sex ratio. The offspring sex ratio of 2 day-old females was significantly lower than 1,3,4,5 day-old females, which of 3 day-old females was highest. But the offspring sex rate had no significant difference in the different age of females after emergence and mating.For in-depth study of changes in the rule and impact of the key factors on offspring seconary sex ratio of Asecodes hispinarum Boucek and Tetrastichus brontispae, which laid the foundation for the study.