| The human hepatocarcinoma cells (H1-JCC-9724-p) were inoculated to different sites of three different immunodeficient mice and the immune normal mice -BALB/c,then the characteristics of the tumor growing were observed, and the T cell percentage and antibodies in mice were assayed; also the lymphocytotoxities of spleens to the hepatocarcinoma(target)cells were detected,so the model characteristics of tumor growing in these mice,and the correlation of the growth and metastasis of the hepatocarcinoma with mice inlmunity could been explored. In this experiment,the HFICC-9724-P were maintained in RPMII 1640 medium at 37 c in a fully humidified atmosphere containing 5% co2 in vitro.The cultures approaching 90% confluence were harvested with 0.25%(w/v) trypsin containing 0.02%(wlv) EDTA in 10mM PBS,then were inoculated to the s.c,i.p,i.h of immune normal BALB/c,B lymphocyte deficient CBAIN,T lymphocyte deficient mude,T and B lymphocytes combined immunodefient SCID mice, and also inoculated to s.c of immunoreconstituted BALB/c-PBL-SCID andCBAIN-PBL-.SCJD. After that,the situations of hepatocarcinoma growth and metastasis were surveyed. The results demonstrated that there were no growth in any sites of in BALB/c;no growth in s.c and i.p groups of CBA/N,but a little node in liver of i.h group.However,the S.C of CBA/N once appeared carcinoma node ,then gradually disappeared after3 weeks as the inoculation cell numbers reached 2x 107/0.2m1. There were all carcinoma growing in s.c of nude,SCID, CBAIN-PBL-SCID,but,the volumes of which were significant difference.Although there were carcinoma growth in the s.c of BALB/c- PBL-SCJD, the tumor volume gradually disappeared after inoculating the tumor cells 3 weeks.In i.p and i.h groups of nude ,SCID mice,there were tumor growth and extensive metastasis ,while the volume and metastasis and range of tumor in SCID overpassed those in nude. The change of immunity in mice were surveyed after inoculation the carcinoma cells.First, the antibodies were detected in the mice with B lymphocytes every week and the agglutiration reaction of sera of the mice with the carcinoma cells were compared.Second,the spleen lymphocytes of mice were examined for direct cytotoxicity agaist the carcinoma (target) cells. Third,the CD4+and CD8+T cell percentage of experimental mice were assayed. The results showed that the spleen lymphcytotoxic potentiality to the target cells was CBAIN,BALB/c,BALB/c-PBL-SCID in turn,while the splees of control groups and SCID ,nude mice showed no cytotoxicity to target cells.The CD4+ and CD8+T cell percentage changed: CD4+T cell and CD4+/ CD8+ratio evidently decreased,when CD8 T cells percentage changed not much.Agglutination of sera of experimental BALB/c with target cells was strong,next was BALB/c-PBL-SCID and nude.The detected immunogiobulin of BALB/c had a tendency from increasing to decreasing,while that of BALB/c-PBL-SCID gradually increased, which reched mg/mi. Conclusions: 1 .There was 100% hepatocarcinoma growth in SCID and the tumor volume of SCID was larger than that of nude;the ratio and range and quantity of metastasis in i.p group of SCJD were also higher than that of nude.So,SCJD mice are the best model animal for establishing hepatocarcinoma animal model.2.The immunoreconstitution of SCID mice was successful with 8 x 1 O6lymphocyte numbers,Which not only embody the characteristics of two mice immunity agaist hepatocarcinoma ,but the appearing GVI-17D was not... |
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