A Study Of Th DAZ And DAZLA Genes In Spermatogenesis And Variation Of The Serum Hormone Levels In Men With Idiopathic Oligozoospermia And Azoospermia

With the wide use of technology of molecular biology, the study of male infertility has reached the level of molecular, and the hot topic is the study of the relational genes in spermatogenesis. The reason why some people with idiopathic oligozoospermia and azoospermia have spermatogenesis obstruction has relation to the microdeletions of genes on Y chromosome, However, pathogenies in most men with idiopathic oligozoospermia and azoospermia~ have not been clear. Maybe there are other causes that. result in spermatogenesis obstruction. Spermatogenesis obstruction is often accompanied by disturbance of reproductive hormones. Therefore, realization of the change of reproductive hormones of idiopathic male infertility has great significance in the analysis of the causes of male infertility, pathogenesis and theprediction of male fertility. The first part of my thesis is to study the role of DAZ and DAZLA genes in spermatogenesis. We use polymerase chain reaction (PCR) technology to screen for the DAZ and DAZLA locus in 20 men with idiopathic azoospermia, 18 men with idiopathic oligozoospermia and in 20 normal fertile men. The results are as followings: 1. There is a close relationship between the DAZ gene and spermatogenesis. DAZ gene plays an important role in pathogenesis of azoospermia -4- and oligospermia with idiopathicity. 2. There is a relationship between DAZLA gene and spermatogenesis. At the beginning of spermatogenesis, the mutation of DAZLA gene may be one of the causes of azoospermia. The second part of the thesis is to study the variation of the concentration of Serum FSH~ LU and T, which are measured in 25 men with idiopathic oligozoospermia and 30 men with idiopathic azoospermia and in 20 normal fertile men through radioimmunoassay. Results are as followings: I. Compared with normal group, the levels of serum FSH and LH in men with idiopathic azoospermia increase significantly (P<0.Ol) while the levels of serum T and T/LH ratio decrease significantly (P<0.0 1), 2. Compared with normal group, the levels of serum FSII and LU in men with idiopathic oligozoospermia increase (P<0.05); T levels are normal; TILL-I ratio decreases (P<0.05). It is suggested that there exist impairment in the function of Leydig cells in addition to functional obstacle of spermatogenesis in men with idiopathic oligozoospermia and azoospermia. FSI-I is the credible evidence of predicting spermato genesis æ…ºunction. T/LU ratio is the sensitive criterion of reflecting mild variation of Leydig cell function.