Therapeutic Effect Of Basic Fibroblast Growth Factor On Experimental Acute Edematous Pancreatitis In Rat

Basic fibroblast growth factor (bFGF) is one of the mitogens that have direct trophic effects on acinar cells as well as facilitate endothelial proliferation and angiogenesis, which facilitates the repair of damaged pancreatic tissues. So it provides a new strategy of pancrestitis therapy. We studied the therapeutic effects on actue pancreatitis in the experimental model of acute edematous pancreatitis in rat aiming at the absence of similar report in our country.Materials and Methods1. Animals and group classification30 Wistar (Sprague-Dawley ) rats were randomized in three experimental groups with 10 each. AEP models were firstly induced by intravenous injection of bombesin and secretin in Group II and Group III while contra! (Group I) was intravenous injecting saline. BFGF was administrated intraperitoneally in Group III. After 24 hours, 5-bromo-2'-deoxyuridine(BrdU) was injected intraperitoneally in Group II and III to examine the DNA synthesis of acniar cells2. Parameters and indexBlood samples were collected for determination of seurm amylase and lipase. And the pancreas were for the pancreatic wet/dry weight ratio detection and for the histological examination. DNA synthesis in acinar cells was determined as the uptake of BrdU, and BrdU labeling acinar cells were also examined with imrnunohistochemical staining.3. Statistic methodStatistic significance of the results was evaluated using Student's t test in personal computer with software of SPSS 10.0 for Windows.Results and Discussion1. There is a significant difference hi result of serum amylase, lipase and the pancreatic wet/dry weight ratio on bFGF-treated pancreatitis rats thanpancreatitis rats without bFGF treatment. (PO.01)2. In the histologic examination of staining with H.E., bFGF found remarkably reduced tissue edema and vacuolization of acinar cells in rats of group treat with bFGF compared with that of pancreatitis group without bFGF treatment.3. The number of BrdU labeling in the nucleus was remarked increasing onbFGF-treated pancreatitis rats than pancreatitis rats without bFGF treatment. And there was a significant difference. (P<0.01)ConclusionThe result of the study has demonstrated the effective therapeutic effects on acute edematous pancreatitis rats, which induces proliferation of immature acinar cells, facilitates the repair of damaged pancreatic tissues and reconstitutes tissue integrity. In brief bFGF plays a promising role in preventing the aggression of acute pancreatitis. Treatment with bFGF may represent a new therapeutic concept for patients with acute pancreatitis.