Research On Candida Species And Drug Resistance In Vulvovaginal Candidiasis

Candida species may cause opportunistic infections. Superficial Candida! infections affect female genital tract, oropharynx in children and old men.Systemic Candidal infections are directly related to the growing population of immunocompromised individuals, resulting from changes in medical practice such as the use of immunosuppressinve drug in organ transplantation and intensive chemotherapy in tumor patients. The aged, diabetes mellitus and other diseases which cause immunosuppression have also contributed to this problem.Therefore, with the aging of population, the increase in the treatment level of the organ transplanation and anti-tumor, the increasing number of AIDS patients and drug addicts, the opportunistic infection of corresponding Candida species may be one of the main factors threatening at梤isk hosts, and may be the chief dying cause of the aged and other at梤isk hosts and the main goal to control nosocomial infections. Antifungal agents are not properly used for treatment or prophylaxis of candidal infections. This practice is most likely responsible for the increased isolation of resistant strains of Candida. The molecular mechanisms of resistance to antifungal is complex and various. There are more and more the incurable cases and cases of recurrence, which makes the antifungal treatment even more difficult. \ ulvovaginal candidiasis is a common disease in obstetrics and gynecologyclinical practice. Approximately 75% of women will have a vaginal infection episode during their life span, and a small percentage of patients (approx.5%) will show a chronic course, characterized by a relapsing vaginal candidiasis. The widespread introduction of numerous highly effective topical and oral systemic azole agents results in a comparable higher cure rates in vulvovaginal candidiasisis. However, it is reported that the increased use of antifungal in recent years is most likely responsible for the increased isolation of resistant strains. A number of findings indicate the percentage of Candida strains with decreased susceptibility did increase from 6% to 36%. A gastrointestinal or skin reservoir is a possible source of vulvovaginal candidiasis. The analysis of the big exponent of Candida species and its drug resistance in vulvovaginal candidiasis may be a good start to research on human Candida! infection and its drug resistance and contribute to the trent research on the change of human Candida species and is helpful to the analysis of potential pathogen which bring about systematic Candida! infection under the circumstances of other pathological situation such as organ transplantation and anti-cancer treatment. The purpose of the study is to throw some light on Candida species and drug resistance in vulvovaginal candidiasis and forthermore provide reference information for understanding other bodily Candida species.From 2000.1 to 2002.2, one thousand and thirty-three doubtful patients with vulvovginal candidosis admitted to our out-patient clinic. One thousand and four cases with vulvovaginal candidosis were finally diagnosed by culture. The related historic characteristics were recorded, including the infectious history of vaginal Candida, the use of antibiotics especially prior antifungal exposure, and whether vaginal douching using antiseptic-containing douche products was frequently used. Mycological cultures were grown on Subouraud glucose-agar and, if positive, fungal indentification is needed with the VITEK 32 system ( BioMerieux ) .ATB FUNGUS was used to detect the susceptibility of 1004 .Candida strains to 6 antimycotis (5-fluorocytosine xamphotericin B^nystatin xketoconazole ^miconazole^econazole).Results;1013 Candida strains were taked from patients with vulvovaginal candidosis and six isolated Candida spp.were identified, including C. albicans, C. glabrata. C. tropicalis, C. parapsilosis, C. Krusei, and Candida lambica. Among them, 1004 clinical strains of Candida were examined for the susceptibility to 6 antimycotis. 419 patients were nonrecurrent Candida vulvovaginiti...