| The pathogenesis of tumour is associated intimately with body s immune state.In fact, in normal body, 107-109 cells are undergoing mutation everyday. Tumour cells with malignant epitope are produced but usually tumour doesri t appear. An important reason is that the body s immunosurreillance can eliminate these cells. When the increase of tumour cells surpass the limit of immunosurreillance, tumour will be formed.Bing responsible for transcriptional actively, the argyrophilic nuclolar regions (Ag-NORs) are acidic nonhistone proteins and are identified by a one-step silver staining technique. The argyrophilia appears to reflect rDNA transcription activity, which has been correlated with proliferative activity of cells. There are many studies about Ag-NORs on tumours, indicated that was correlation withPCNA, Ki-67; benign and malignant tumour; potentially malignant lesions;grade of tumour.T lymphocytes are the major immuno-active cells, which participate in the immunoreaction against tumour. Not only do they play a great role in cell immunity, but also do they play role in immunoregulation. The regulation is realized by its subgroup cells.On the surface of red cell, there are many substances participating in the immunoreaction, such as CR^ CRs> CDs8> CD.s9> DAF^ SOD enzyme,etc. They all take part in the complex regulation of immunonetwork. Red cells have the function of immune adherence. They can adhere, transport and eliminate immune complex in circulation. They play role in the regulation of immune activity of T, B> NIC-. LAK cells and phagocytes, too. So red cells are an important part in the body' s immunity against tumour.Nucleolar organizer regions and T lymphocyte subgroups (including CD3 , CD4*> CDg")of peripheral blood and erythrocyte immune function (including RBC-CsbRR and RBC-ICR) were measured in 86 patients with malignant tumours (such as liver,osteoma ,esophagus).The methods of Ag-NORs KL image-analysis,direct immunoflurescent staining technique with flow cytometry(FCM) and immune adherence rosette were used in the study.The results showed that:(l)The value of I.S% was lower in cancer patients than those of 42 healthy control subjects (PO.01). (2) The variation of I.S% ratio was significantly relative with clinical stages and was not relative with pathological classification in liver. (3) The value of I.S% was lower in liver patients than those of 22 cirrhosis and 42 healthy control subjects (P<0.01) and was stitistical different between cirrhosis and healthy control (P<0.05). (4) The value of I.S% was significantly relative with tumour transformation.^) The values of CDs*, CD4", CD47CDs~were lower in cancer patients than those of 42 healthy control subjects (P<0.01) and the value of CDg+ was not statistical different between two groups CP>0.05).The values of RBC-C3bRR was lower and RBC-ICR was higher in the group of cancer patients as compared with the group of 42 healthy control subjects (P<0.01). (7)The value of I.S% significantly correlated with the CD4 /CD8~ ratio in the cancer groupe (r=0.713, PO.01) and the value of I.S% significantly correlated with the value of RBC-C3bRR in cancer groupe(r= 0.503, P<0.01).The decreased I.S% ratio indicated that the cell proliferation was depressed. The I.S% ratio was a sensitive index of reflecting cell proliferation.The decreased CD4+/CDg^ ratio indicated by T lymphocyte was depresed and balance was interrupted. The decreased value of RBC-C3bRR and increased RBC-ICR demonstrated that RBC-Csb receptor activity and 1C elimination was significantly depressed in the patients with malignant tumours. The above effects result in the whole immune system function and the anti-tumour-immune activity depressed.The Index I.S% significantly correlated with the immune function of body. The detected of I.S% ratio has important significance in clinical diagnosis and threapy evalueated and prognosis assessment of malignat tumourand a simple and exact methed. |
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