Analysis Of The Expression Of Tenascin, CD34, Ki67 And Their Relationship With Human Glioma Invasion And Proliferation

Analysis of the expression of tenascin, CD34, Ki67 and their relationship with human glioma invasion and proliferationInvasion and metastasis are the two characteristic of the maligand tumor, what's more, they are direct cause of the patient death. In our study, in situ hybridization and immunohistochemistry techniques were used to investigate the relationship of microvascular density (MVD) with the invasion and metastasis in the progression of the human glioma invasion. The expression, distribution, function of Tenascin, CD34 and Ki67 were studied in different degrees of gliomas, meningionas and brain metastases. We analysed the relationship among Tenascin, Ki67 proliferating index (PI) and MVD, explored the role of Tenascin, CD34 and Ki67 in the invasive progression of the human gliomas.(1) Distribution and function of Tenascin: The expression of Tenascin mRNA and proteins were found both in gliomas and brain metastases, and they expressed in the same regions. No expression of Tenascin was found in meningions. Image analysis was applied to deal with the results of in situ hybridization and immunohitochemistry. We found that the expression of Tenascin in brain metatases was significantly higher than that of in gliomas (PO.01). The expression of Tenascin increased with the degree of tumor malignancy. The expression of Tenascin was closely related with the clinical staging of the gliomas.(2) The relationship of MVD with the invasion: CD34 monoclonal angibody was used in Immunohistochemistry SABC. MVD was counted under microscope (X200) .We found MVD was the highest in GBM, followed by glioma III,then-4-glioma II and glioma I . In brain metastase MVD was significantly higher than that of glioma and meningioma (P<0.01).(3) The relationship between Ki67 LI and invasion: Monoclonal antibody Ki67 was used with immunohistochemistry SABC. The results were indicated in labeling index (LI). We found that was highest in brain metastases, in addition, Ki67 LI increased with the tumor malignancy, expression of Tenascin and Ki67 LI correlated positively with each other(/-=0.748, P<0.01).Conclusion:1. Tenascin was highly expressed in the cytoplasm of GBM, Tenasein might promote the gliomas angiogenesis and play a role in the invasion of gliomas.2. MVD increased with the tumor malignancy of gliomas. MVD was the highest in brain metastases, and related closely with the invasion of gliomas.3. Ki67 LI increased with the tumor malignancy of glioma, Ki67 LI was the highest in brain metastases. Thus, Ki67 LI had some relation with the grading of the glioma, and might be useful in distinguishing brain metastasis from primary brain tumor.4. Tenascin, CD34 and Ki67 correlated each other. It suggested that Ki67 may participate in the invasion of gliomas.