An Experimental Study Of Interventional Therapy Of Minipump For The Treatment Of Steroid-Induced Necrosis Of Femoral Head

Objective: To analyse preliminarily the relationship between systemic pre-thromboticstutes ,disorder of lipometabolism function of vascular endothelial cell and steroid-induced necrosis of the femoral head, investigate the mechanism of steroid-induced necrosis of the femoral head, and find the experimental evidence for interventional therapy by nitroglycerin as exterior nitric oxide donor used in steroid-induced necrosis of the femoral head, in order to supply effective evidence for the clinical practice early.Methods: 30 rabbits were divided randomly into 3 groups. Group A were composed of six control animals. Group B were composed of twelve animals that were treated with a combination of sterile horse serum and methylpoednisolone. They received l0ml/kg of sterile horse serum intravenously twice at three weeks intervals. Two weeks after the second injection of sterile horse serum, the 40mg/kg/day of methylpoednisolone acetate were injected intraperitoneally for three conseoutive day. Group C were composed of twelve animals too. After given the same practice as Group B, they received the two weeksinterventional therapy of minipump by nitroglycerin as exterior nitric oxide donor. Blood samples were collected from heart puncture immediately according to the following time span: before the injection of sterile horse serum, after injection of methylpoednisolone and after therapy completely. OX-LDL,GMP-140 and NOS were measured with ELISA. The histopathological changes of femoral head of all rabbits were observed after the end point of experiment.Results: There were significant difference about OX-LDL,GMP-140 and NOS in Group B, so did Group C(p< 0.01). There were significant difference about OX-LDL, GMP-140 and NOS after model finished successfully, so did after therapy completely (p<0.01).Conclusion: Steroid caused the disorder of lipid metabolism that lead to the injury of vascular and disorder of systemic coagulation, which play an important role in steroid-induced femoral head necrosis .Interventional therapy by nitric oxide can improve the blood supply of steroid-induced femoral head necrosis, which is an effective method in the treatment of steroid-induced femoral head necrosis .