Cotransplantation Of Mesenchymal Stem Cells Resulted In Enhancement Of Hematopoitic Reconstitution After HSCT

Objective To explore the hematopoietic reconstitution potential of mesenchymal stem cells after Hematopoietic stem cell transplantation(HSCT) . Methods We cotransplanted human derived bone marrow mesenchymal stem cells(MSC) with or without Hematopoietic stem cell transplantation(HSCT) into lethallyrradiated SCID mice. Results All mice of group 1 died in 4-5 days post irradiation following transplantation, the mice in group 2 all died in 10 days.while the mice from other two groups survived. The time course of hematopoietic reconstitution was then observed. The initial time of peripheral blood MNC count recoverey had no differencebetween the two groups. Interestingly,there was significantincrease of MNCs in peripheral blood of group 4 by week 3. Furthermore, by week 7, PCR analysis was performed using peripheral blood cells to determine if any human-derived cells were present.human-derived cells were found in both of the two groups.FACS was used to detect the chimerism ,higher level of chemirism was found when more MSCs were cotramsplanted. post transplantation. Both of the two groups have good survival and a low transplantation related mortality. Conclusion These results indicate that cotransplant with mesenchymal stem cells can marly re-establish hematopoiesis in lethally irradiated SCID mice.and reduce treatment related mortally. This study would provide an alternative transplantation treatment for malignancy.