| Objective: To explore the action of IGF- II ,TGF a and EGF in liver cirrhosis and primary hepatocellular carcinoma. We evaluated serum levels of IGF- II ,TGF a and EGF in the patients with liver cirrhosis and primary hepatocellular carcinoma and studied the relationship among serum levels of IGF- II ,TGF a , EGF and some biochemical parameters reflecting the severity of hepatic function. We preliminarily explored the relationship among the serum levels of IGF-II ,TGF a and EGF .Methods: 54 hospitalizing patients with liver cirrhosis (46 men and 8 women, aged 25-71 years, average age 53 years), 32 patients with primary hepatocellular carcinoma(26 men and 6 women, aged 29-72 years, average age 56 years) and 32 sex, age-matched healthy subjects(26 men and 6 women, aged 29-72years, average age 56 years) were included in the present study. The diagnosis of liver cirrhosis had been made on the basis of clinical ,biochemical and serological parameters using standard techniques including ultrasound and computed tomography. 10 patients were assessed as Child A stage, 22 Child B stage and 22 Child Cstage. Cirrhosis was decompensated in 37, compensated in 17 patients. Primary hepatocellular carcinoma (PHC) was confirmed on clinical grounds including serum a-fetoprotein(AFP) and findings of ultrasonography, computed tomography, magnetic resonance imaging, angiography and /or histopathology. PHC patients with distant metastasis were in 8(25%), without distant metastasis were in 24(75 %). All subjects had no other diseases and did not take the medicine which could produce acute liver damage . Venous blood samples were drawn after an overnight fast at 7-8 AM, centrifuged and then serum was stored at -80 癈 until assayed. The levels of IGF-II,TGF a and EGF in blood were determined by radioimmuno-assay(RIA) after complete recovery.Results : 1. In cirrhotic group, the serum levels of IGF-II (0.58 ?0.20ng/ml), TGF a (5.99 ?1.91pg/ml)and EGF(581.79?88.06pg/ml) was higher than that in control group(p<0.01, p<0.05, p<0.01). The serum levels of IGF-II,TGF a and EGF in patients with PHC(0.78?.23ng/ml, 7.34 ?1.86pg/ml and 809.85 ?224.23pg/ml respectively) were much significantly higher than that in cirrhotic group and control group(p<0.01). 2. In cirrhotic group, a negative correlation was found between IGF- II and albumin, A/G(r=-0.446, p<0.01; r=-0.437, pO.OOl). A positive correlation was obtained between IGF- II and prothrombin time(PT) and Child-Pugh score (r=0.49,p<0.01;r=0.33,p<0.05). there was no significant correlation between TGF a and ALT, AST, total bilirubin(TB), albumin, A/G, PT and Child-Pugh score(p>0.05). serum EOF levels in LC exhibited a significant positive correlation with total bilirubin , prothrombin time and Child-Pugh score (r=0.286, p<0.05; r=0.275, PO.05; r=0.392, p<0.01). 3. In cirrhotic group, the serum levels of IGF- II ,TGF a and EGF had a trend, that is, Child A0.05), the serum levels of IGF- II and EGF were associated with the Child-Pugh classification (p<0.05). 4. There was a significant positive correlation between IGF-II and AFP in PHC (r=0.878, p<0.01). No significant correlation was found between TGF a ,EGF and AFP(p>0.05). In 32 PHC, the positive rate of AFP, IGF- II, TGF a and EGF were 59.4%, 68.8%, 46.9% and 68.8% respectively. The difference among the positive rates was no significant (x2=4.332, df=3, P>0.05). 13 PHC were found to be negative to AFP, in which IGF- II was positive in 4 PHC (30.8%). In PHC, the serum levels of TGF a in patients with distant metastasis(8.75?.59pg/ml) was much significantly higher than that in the patients without distant metastasis(6.87 + 1.73pg/ml, p<0.05). 5. There was no significant correlation between IGF- II and TGF a , IGF- II and EGF, TGF a and EGF in LC, PHC and control group(P>0.05).Conclusion: In this study, we found that: 1. The levels of IGF- II ,TGF a and EGF in blood of patients with liver cirrhosis were higher...
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