| Cervical cancer is a kind of women malignant neoplasm with a high incidence of disease, its occurrence and development are complicated, gradual biological behavior with multiple factors and procedures including all kinds of oncogenes activation and inactivation of anti-oncogene. In recent years, with the increase of high incidence of sexually transmitted disease the human papilloma virus(HPV) infection has leaped to first place in the incidence of venereal disease. The incidence of cervical cancer also has increased and prevailed in the young. The study of the relation between the HPV infection and the cervical cancer began in 1970's. With the deveopment of the technology of molecular biology, telomere and telomerase as gene constitution, which can make cells immortalize, began to the realized by the people. Due to their the occurrence, development, diagnosis and treatment of tumor, telomere and telomerase are becoming the new marker of tumorgradually. At present, study of the relation between the HPV infection and the cervical cancer at home and only limits to the correlation between them, and the results are different abroad. We don't have enough knowledge about the mechanism of the HPVig/ig infection leading to the telomerase activity.In view of HPV infection and the expression of telomerase activity are both related to the periodic regulation of cells proliferating cell nuclear antigen (PCNA) is the marker which can reflect cell proliferation. In this study, polymerase chain reaction (PCR) immunohistochemical labeled-streptavidin biotin method (LSAB) are applied in qualitative and quantitative analysis to the alteration of HPVje/ig, PCNA and telomerase, and the influence of HPVi6/i8 infection on PCNA and telomerase in 180 cases of cervical cancer, so that we can understand that HPV]6/i8 infection leading to the alteration of PCNA and telomerase in cervical cancers offers a new clinical marker in judging the occurrence of cervical cancers and giving us prognosis. 180 cases of cervical samples include: 90 cases of control groups (normal cervical: 47, chronic inflammation: 43); 60 cases of cervical intra-epithelial neoplasia (14 cases of CIN- I , 22 cases of CIN-II, 24 cases of CIN-III); 30 cases of cervical cancer (squamous carcinoma: 27, adenocarcinoma: 2, adenosquamous carcinoma: 1; stage I : 9, stage 11:11, stage 111:8, stage IV :2; well differentiated grade :7, moderate differentiated grade :13, low differentiated grade: 10 ; 17 cases of tumor diameter^ 4cm, 13 cases of tumor diameter<4cm;11 cases with pelvic kidney lymph node transfer, 19 cases without ). All of cervical cancer patients didn't accept any chemotherapy and radiotherapy therapy before operation.Resultsl.In the process of cervical lesion controls -*?CIN -*?cancers),the positive rate of HPV16/18 is 12.22% ?8.33%?63.30%; that of PCNA is 6.67%?53.33%?86.67%; and that of telomerase is 0?8.33%?93.33%. Made a comparison between any two of the three, the rate of positive expression is remarkably different (PO.01).2.1n the lesion process of cervical intra-epithelial neoplasia,PCNA and telomerase expression of HPVie/ig-positive group is obviously higher than that of negative group. The difference is remarkable(CIN- I ~ II : PO.05; CIN-III: PO.01). Between the two groups of controls and cervical cancers, HPV]6/i8-positive rate is higher than negative one, but there is no remarkable difference between them (P>0.05).3 .With the aggravation of the cervical lesion, the percentage of positive cells of PCNA and telomerase shows an upward tendency. Making a compairison between any two of the three, there is remarkable difference (PO.05).4. In control groups the percentage of positive cells of PCNA of HPV16/18-positive (x眘) is higher than that of HPVi6/i8-negative, but there is no remarkable difference between them (P>0.05). While, in the groups of CIN- I ~ II ,CIN-III and cancers, thepercentage of positive cells of PCNA of HPV(6/18-positive is obviously higher than that of HPV!6/i8-negative (CIN- I ~ II : PO... |
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