Expression Of VCAM-1 And MMP-9 In Placenta Of Pregnancy Induced Hypertension Complicated By IUGR

Pregnancy induced hypertension is a important cause of intrauterine growth retardation.. It is generally identified that shortage of placenta blood and letdown of placenta function come from uterus vessel hyperkinesia in PIH can lead IUGR. In this pathologic course,it is few investigated now that what kinds of genes are operating and which pathologic change appear in placenta. Basing on the theory of vessel endodelium scathe and blood shortage of placenta-syncytiotrophoblast,we study the expression of VCAM-1 and MMP-9,which act import role in the course of vessel endodel ium scathe and placenta development,and discuss their role in PIH complicated by IUGR.Objective:1 To study the pathological change by microscope and electron microscope and discuss the pathological reason of intrauterine growth retardation originned from PIH.2 To study the expression of vascular adhesion molecule-1 (VCAM-1)and matrix metalloproteinase-9 (MMP-9) in placentaes of pregnancy induced hypertension complicatedby intrauterine growth retardation and evaluate their role in the disease.3,To study the relation between the expression of VCAM-1,MMP-9 and the pathological change in placentas of intrauterine growth retardation originned from PIH.Methods:1 The placental samples come from 30 pregnancies with PIH complicated by IUGR patients,25 PIH patients without complication,28 IUGR patients without PIH and 30 normal pregnant women . Tissue sections were dealled with by HE stain and PAS stain and then observed by microscope to find the morphological change. To get 5 samples another from PIH complicated by IUGR group and give an observation by 500H model electron microscope.2 The placenta samples were collected from 30 pregnancies with PIH complicated by IUGR patients,28 IUGR patients without PIH and 30 normal pregnant women. Immunohistochemical analysis was employed to demonstrate the postitive expression of the VCAM-1 in placenta vascular endothelium and syncytiotrophoblast as well as the MMP-9 in syncytiotrophoblast of these samples.3 The postitive expression of the VCAM-1 in placenta vascular endothelium and syncytiotrophoblast as well as the MMP-9 in syncytiotrophoblast were analysed in placentas who had pathological change and no pathological change.Results:1 There are significantly pathological change in PIH complicated by IUGR:(1) Villous stroma fibrosis and fibrinoid necrosis;leucocyte soakage;(2) villous vascular decrease and gore,blood vessel velamen absence;(3) cytotrophoblasts hyperplasia,villous syncytial knots manifold and basal lamina thicking. The different pathological changes in PIH complited by IUGR compared with PIH are blood vessel velamen absence and basal lamina thicking. Villous stroma fibrosis and fibrinoid necrosis,villous vascular gore,villous syncytial knots manifold and basal lamina thicking are significantly different when it compared with IUGR without PIH group. The especially pathological changes in IUGR with PIH are villous stroma leucocyte soakage,villous vascular increase and villous syncytial knots shortage. The syncytiotrophoblast ultrastructure marked change,decidua spiral arteries endodermis damnification,arteries cavity straitness and part of arteries extremity obstruction were finded by electron microscope.2 In PIH complicated by IUGR placentas,there was markedly higher expression of VCAM-1 in the decidual vascular endothelium than those in normal pregnancies (P<0. 005). There are not marked difference in former two groups when they compared each other. Morever,the positiveexpression increased as the PIH advanced in PIH complicated by IUGR group. The postitive expression of the VCAM-1 was markedly higher in placenta villous capillary vessel of PIH complicated by IUGR group and IUGR without PIH group (P<0. 05),but there are not marked difference between them. There was markedly lower expression of VCAM-1 in syncytiotrophoblast in all IUGR placentas and the positive expression extremely decrease as the PIH advanced in PIH complicated by IUGR group.3 The p...