The Effect Of The Micrometer Anticancer Compound Ⅰ In Inhibiting Mice Carcinoma: A Study In Vitro And Vivo

Object: There were many methods and abundant clinical experience in treatment of tumor with Chinese traditional drugs. Anticancer compound I , a folk prescription, can inhibit the growth of carcinoma significantly. By pulverization technique, anticancer compound I was made into micrometer preparation in which the diameter of drug granules were 1.0um~2.0um. The micrometer Chinese traditional drugs not only had the main function of the original herbs but also improve its inhibitory effect and biological,utilization ratio. Therefore, the present experiment is intended to study the effect and mechanisms of micrometer anticancer compound I in inhibiting mice carcinoma in vitro and vivo.Methods:1) The model of mice bearing carcinoma was established by inoculating sarcoma-180 cells into the subcutaneous tissue or the abdominal cavity of the BALB/C mice. 2) In the inhibitory experiment of the solid carcinoma in vivo, the BALB/C mice bearing sarcoma-180 cells were divided into six groups: high dose group of micrometer Chinese traditional drugs; medium dose group of micrometer Chinese traditional drugs; low dose group of micrometer Chinese traditional drugs; MMC group; extraction group of anticancer compound I ; control group. The various drugs and natural saline were perfused into micestomach at the 5th day after inoculating sarcoma-180 cells. Then five days later, execute the mice, cut the tumor, weigh it and calculate the inhibition ratios of the tumor weight. 3) In the inhibitory experiment of the ascites carcinoma in vivo, the BALB/C mice bearing sarcoma-180 cells were divided into six groups:high dosegroup of micrometer Chinese traditional drugs; medium dose group of micrometer Chinese traditional drugs; low dose group of micrometer Chinese traditional drugs; MMC group; extraction group of anticancer compound I ;control group. The mice were injected the various drugs and natural saline into the abdominal cavity at the 4th day after inoculating sarcoma-180 cells. Count the survival period of tumor-bearing mice and calculate the prolongation ratios of life.-span. 4) In the inhibitory experiment of the sarcoma-180 cells in vitro, the sarcoma-180 cells diluted in natural saline were put into 12-well plate and treated respectively with the different dose micrometer Chinese traditional drugs, extraction of anticancer compound I and natural saline. After incubated at 37 癈 for 15 minutes, the cells were dyed with Trypan blue. Count up the death ratios of each group cells. 5) In the experiment of apoptosis examination, the experiment group mice were injected with the micrometer Chinese traditional drugs (32.4mg/kg) into the abdominal cavity and the control group mice were injected with natural saline (0.5ml) into the abdominal cavity at the 3rd day after the BALB/C mice inoculated with the ascites carcinoma cells,. Three days later, the ascites was draw and examined by the transmission electron microscope and the flow cytometery.Results:1) In the inhibitory experiment of the solid carcinoma in vivo, the distinction between experiment group and control group had a significantly meaning(P<0.01) by analyzing average weight of the each group solid carcinoma, which indicated that the drugs of experiment group could inhibit sarcoma-180. In micrometer Chinese traditional drugs group, the inhibitory effect and the inhibitory ratios rose with the increase of dose. 2) In the inhibitory experiment of the ascites carcinoma in vivo, the average survival time of each group mice bearing sarcoma-180 cells were counted up. It indicated that each dose micrometer Chinese traditional drugs and MMC could inhibit sarcoma-180 cells and prolongthe life of mice bearing sarcoma-180 cells significantly(P<0.01), The distinction between each dose group of micrometer Chinese traditional drugs and MMC group had no significantly meaning(P>0.05). The distinction between the group of micrometer Chinese traditional drugs and the extraction group had a significantly meaning(P<0.01). Each dose group of micrometer Chinese traditional drugs could...