| Despite its decline in incidence in recent decades, gastric cancer remains one of the most common diagnosed malignancies in the world and is the leading cause of cancer death in China. Jiangsu Province is one of the highest-risk areas of gastric cancer in our country, with the mortality rate of 33.32 per 100 000. To date, the etiology of gastric cancer is unclear. To emplore the genetic risk factors and environmental risk factors, a molecular epidemiological study was conducted in Jintan and Chuzhou county of Jiangsu provence, two of the higher-risk areas of gastric cancer. The result will offer theoretical evidences for instructing the primary prevention for population at high risk.Multivariate Logistic regression analysis showed the history of chronic gastritis(OR=2.312), gastric ulcer(OR=2.855) and the family history of gastric cancer(OR=2.393) were the independent risk factors for gastric cancer. Being angry frequently (OR=1.693), Irregularity in diet (OR=2.137) and intaking hard food (OR=1.736) can increase the risk of gastric cancer, tea drinking (OR=0.629) was a protective factor. Logistic regression analysis showed the odds ratios of history of chronic gastritis, gastric ulcer, Irregularity in diet, being angry frequently were increased significantly when combined with family history of gastric cancer, compared to the ORs of each single factor respectively.E-cadherin(CDHl) is an important member of the cadherin family localized at the epithelial cells. CDH1 plays a critical role in the establishment and maintenance of intercellular adhesion, cell polarity, and tissue architecture. Germline mutation of the CDH1 has been found in familial diffuse gastric cancer. Dysfunction of E-cadherin due to mutation of CDH1 was also found in diffuse-type gastric cancer. To explore theassociation between the -160 C→A promoter polymorphism of CDH1 and gastric cancer risk, we undertaken a population-based case-control study using PCR-RFLP.There were three genotypes of CDH1 resulting from digestion with restriction enzyme Hinc II: a wild-type homozygote CC, a heterozygote CA, a mutant type homozygote AA. The present study showed frequencies of CC, CA , and AA genotype were 57.8%, 36.4% and 5.8%, respectively in cases; and were 58.2%, 34.9% and 6.9% respectively in controls. There was no significant difference in the frequencies of CC, CA, AA genotypes between cases and controls (P=0.87). No significant was found association between CDH1 polymorphism and the susceptibility to gastric cancer, after stratified by sex, age, area, smoking, alcohol use, H.pylori infection and family history of gastric cancer in the multivariate logistic regression analysis. The present study showed the CC genotype of CDH1 was the dominant one (58.2%) in Hans in south of china. The frequencies of CDH1 in this Chinese population were similar to that in Europeans and different from Japanese and Taiwanese. The frequency of CC genotype in Japanese (69.4%) was higher than other populations, but the frequency of AA genotype was only 3.4%, which was lower than other population. In Taiwanese, the frequency of CC genotype (OR=42.3%)was lower than that of CA genotype (OR=48.0%) , which differed from other enthic groups.O6-methylguanine is one of the most critical lesions leading to the induction of mutations. O6-methylguanine-DNA methytransferase (MGMT) palys an important role in repair of alkylating agent-induced DNA damage by transfering the methyl group from O6-methylguanine of DNA to its own cysteine residue and prevents the carcinogenesis of alkylation. It was reported that there were some single-nucleotide polymorphisms (SNPs) in MGMT gene which decrease the MGMT activity and increased the susceptibility to cancer. So we undertaken a population-based case-control study using PCR-RFLP to explore therelationship between the two polymorphisms in exon 3 and the susceptibility to gastric cancer.This study showed that frequencies of Ieu531eu genotypes CC, CT , and TT genotype were 75.5%, 21.4% and 3.1%, r... |
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