Studies On Biologic Characteristics And Main Active Compositions Of Phellinus Igniarius

In order to devolping and utilizing Phellinus igniarius resources, the main biologic characteristics and polysaccharide of Phellinus igniarius were studied. Meanwhile the optimum method suitable for mass production of polysacchride was established. The basis data on studying active compositions, developing new medicine and culturing fruit bodies from mycelia of Phellinus igniarius were provided.Lately research indicated that Phellinus igniarius had the activity of antitumor and the polysaccharide content of mycelia was 37.5%. But because of having not mastered the growth conditions of mycelia and not succeeded in culturing fruit bodies, it was retarded the pace of developing and utilizing the new medical source.The biological characteristics of mycelia from Phellinus igniarius and culture media were studied. Two kinds of culture media were suitable for the growth of mycelia. The result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia, and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation. Meanwhile the depiction of mycelia could contribute the identification among species.Eight extracellular anzymes of mycelia from Phellinus igniarius cultured by shaking were studied for the first time. The extracellular anzymes were measured within 20 days. The result showed that the fruit bodies of Phellinus igniarius could be cultured because of having the whole enzymic system. The peak times of amylase, CMC enzyme,cellulose, laccase, guaiacol oxidase and polyphenol oxidase were the 10th day, the 12th day, the 12th day, the 14th day, the 16th day and the 16th day respectively. It indicated that Phellinus igniarius has the capability of discomposing amylum, cellulose and lignose. The peak time of protein and reductic saccharide were the 14th and the 16th. It showed that optimum time of collecting protein and reductic saccharide were the 14th day and the 16th day respectively.Polysaccharide of mycelia from Phellinus igniarius cultured by shaking were studied. Lg9(34) orthogonal experiment were carried out to find the optimum method of extracting polysaccharide from mycelia. The magnitude of range indicated that the factors which effected extracting ratio were times, time, temperature and proportion. The optimum condition was: proportion 1:50, temperature 90, time 180min and two times. A mixed method composed of proteinase, trichloroactic acid and Sevag was established which had high efficiency and spared sources. It could make the content of protein in polysaccharide under 4%.A substance extracted from mycelia with benzinum was studied for the first time. Five compositions were found: butylated hydroxytoluene, n-hexadcanoic acid, 9,12-octadecadienoic acid methyl ester , eicosane and hexacosane.The paper provided scientific basis data for researching mainly biologic characteristics and active compositions of mycelia from Phellinus igniarius cultured with solid and liquid culture media. It provided information in cultivation of its mycelia and fruit body. Also it had practical significance for accelerating the speed of research, creating economic and social benefits..