| Ischemic heart disease is hazardous to human health, and is main cause of human death. The incidence of ischemic heart disease is increasing and the onset ages become younger and younger. Qidan Tongmai tablet (QDTMT), a kind of traditional Chinese medicine, was established on the theory of Oixue and on the years of clinical experience. Clinical evidence proved that QDTMT showed preventive effects on ischemic heart disease, but the molecular mechanism was still unknown, which impeded its application. Therefore, macroscopic observation and immunohis to chemisty me thods were employed to reveal its mechanism. Experiment I Effects of QDTMT on experimental acute myocardial ischemia rats and on the expression of VEGF and bFGF Material and methods48 SD rats were randomly divided into blank control group, model group, QDTMT min and QDTMT max. Saline were given to blank controlgroup and model group at 10mL/kg weight; Suspension of QDTMT were given to QDTMT min and QDTMT max at dose of 1.0g/kg weight and 2.0g/kg weight. After 14-day administration of QDTMT, isoproterenol (4mg/kg . d) was subcutaneously injected to cause myocardial ischemia. Electrocardiography was used to monitor myocardial ischemia. HE staining was employed to analyze the pathological damage in myocardium of heart apex and ABC staining to detect the expression of VEGF and bFGF in the myocardium. According to weinder's method, two slides of one sample were selected for counting of positive cells. ResultsElectrocardiographic monitoringTwo rats died of acute pulmonary edema were droped out. Compared with the electrocardiography before ISO injection the ST segment was changed clinically in all groups, which proved that the animal model was successfully copied. Pathological damageNo ischemia damage was found in blank control group. Necrosis was found in QDTMT max rats. In model group and QDTMT min, II and III degrees were main damage. The difference of pathological damage was significantly by Kruskal Wallis Test(x2=35.65, p<0.01). Expression of VEGF and bFGFNo expression of VEGF and bFGF was detected in blank control group. However VEGF and bFGF were detected expressed in all ischemia groups. VEGF expressed highly in QDTMT max(25.0+3.1/100cell), and 20.0+ 4.0/100cell, 5.0+ 2.3/100cell in QDTMT min and model group. The difference was significantly by analysis of variance, and LSD test showedthat the difference was nonsiganificantly in the two groups used QDTMT.However, the expression was siganificantly higher in groups used QDTMT than in model group.The expression mounts of bFGF in groups were 15.0+4.9/100cell, 16.0 +4.3/100cell and 3.0+1.0/100cell in QDTMT max, QDTMT min and model group respectively. Statistic analysis indicated that mounts of expression in model group were lower than in QDTMT min and in QDTMT max (p<0.01). However, there is no significance in the two groups used QDTMT by LSD statistic analysis (p>0.05). CONCLUSIONQDTMT could ameliorate myocardial ischemia and promote the expression of VEGF and bFGF, which was indicated that were the possible mechanism of antimyocardial ischemia.Experiments II Effects of QDTMT on the expression of VEGF and bFGF in primarily cultured cardiac myocyteMaterial and methodsPreparation of serum contained QDTMT 30 SD rats were randomly divided into saline group, QDTMT min, and QDTMTmax. QDTMT dosage was same as experiment one. After admistration of QDTMT for 10 days, the rats were killed and blood were draw out and serum were seperated by centrifugation. Primary cultures of myocardium cell Cardiac ventricle muscles sterilely taken out from neonate SD rats were primarily cultured. After 4 days culture, the cells were divided into normal control group, saline group, QDTMT min, and QDTMT max.Each group was divided into two parts and was cultured at normalcondition or at anoxia condition for 24 h. Then ABC staining was employed to detect the expression of VEGF and bFGF. Weinder's methodwas employed to count positive cells. ResultsNo expression w... |
PDF Full Text Request