Experimental Study Of Acupuncture Preconditioning Effect On Ultrastructure And Apoptosis Of Rat's Brain Ischemia

To explore new preventing and cure method of cerebrovascular disease and preventive mechanism on ischemic cerebral apoplexy of acupuncture, this thesis acupuncture before brain ischemia in four vessels occlusion(4-vo) model rats. After ischemia, in cerebral cortex and hippocampal CA1 section use HE staining to observe change of histomorphology, neuronal Nissl's staining to observe neuronal damage, TUNEL method to display apoptosis state, electron microscope to observe ultrastructure. Result :compared with normal group , sham-opration group, the lose neuron in cerebral cortex and hippocampal CA1 section of ischemic group is evident , apoptosis is more evident after 24h, 48h, 72h, compared with ischemic group ,neural damage , dropsy and apoptosis in BIP group , acupuncture preconditioning group and electro-acupuncture preconditioning group is little, recovery of neuron is rapid, electro-acupuncture preconditioning group is perior to acupuncture preconditioning group. Conclusion: pre-acupuncture have preventive effect on neuron of ischemic cerebral cortex and hippocampal CA1 section, pre-acupuncture can prevent apoptosis, pre-acupuncture can act as a method of BIP, can induce BIT and lighten neural damage of ischemic cerebrovascular disease.