| PrefaceRSA is a common pathological pregnancy, which is due to immunology between maternal - fetal interfere, infection, endocrine, even some reasons are unknown. It has been wildly accepted that the immune endurance during the first trimester is important to the maintenance of pregnancy. In normal pregnancy, deciduas are characterized by an altered immune cell profile IL - 4 while RSA show interferon IFN - gamma production pattern. But the mechanism in the alternation of Th0 is not clear. From the studies we can conclude that TCRγδ Lymphocytes can modulate the division, we also found that TCRγδ Lymphocytes increased with the pregnancy. So many researchers make studies on whether there are some relationship between TCRγδ Lymphocytes and maternal - fetal immunology. They found that in contrast to HLA - A and - B Class I antigens in human trophoblast cells non-polymorphic Class I molecules, HLA - G Class Ib, are expressed in extravillous cytotrophoblast and also in endothelial cells of fetal vessels in the chorionic villi . According to our present knowledge, HLA - G presents antigens for TCRγδ T cells and at the same time defends the trophoblast from cytotoxic effector mechanisms. Since polymorphic MHC is absent from the trophoblast, presentation of fetally derived an-tigens is unlikely to be MHC restricted. Most TCRγδ T cells recognize unprocessed foreign antigens without MHC. In the deciduas TCR@ positive cells significantly increase in number and the majority of deciduas TCRγδ T cells are in an activated form due to recognition of conserved mammalian molecules on the trophoblast. Many observations suggest that pregnancy is associated with an altered TH1/TH2 balance. Maternal immune response is biased toward hormonal immunity and away from cell - mediated immunity that could be harmful to the fetus. TCRγδT cells has also been proved to be one of the factors which can change the balance of Th1/Th2. From above ,we can induced that TCRγδT cells play an important role in maternal - fetal immunology.Immunotherapy is useful for RSA . By comparing the value before and after therapy, we found that the level of TCRγδT cells , TCRγδ lymphocyte / CD3 Lymphocyte are significantly higher after therapy than that of the before group . The study showed that immunotherapy could increase the level of anti - husband - antibody in women with RSA, which benefit for the next pregnancy. Increased TCRγδT cells supposed to have some effects on the regulation of maternal - fetal immunology.Material and method1. Patients; All patiences from our department, have at least 2 spontaneous abortions, were excluded chromosome , endocrine disease , As - Ab.ACA.CMV - DNA.VB19 - DNA.TO - IgM and sperm abnormal. Because of blocking antibody negative, they ask for immunotherapy. Blood sample were collected before and 2 weeks after immu-?8 -notherapy.2. Method2. 1 Blocking antibody: In micro - lymphocyte toxin test, the percentage of killing lymphocyte is blocking antibody , @10% is positive , < 10% is negative .2. 2 Immunotherapy : Lymphocyte from husband' s 10mL blood sample were injected into wife's skin , immunotherapy begin 1 month before they plan to pregnancy. Once every week for 4 times. Then they plane to pregnancy under direction. When passing their previous abortion time were considered success.2. 3 Blood sample: Before the first therapy, 2 weeks after therapy, wives 200μL blood sample were collected .2. 4 TCR -y8 T lymphocyte examination : lymphocyte were labeled using anti - CD3 ,anti - γδ antibody, then the two kinds of lymphocyte were examined by FACS.3. Statistic; All the datas were analysed by SPSS[ 10. 0].ResultOf the 15 patients, 11 pregnancy under direction and treatment including 2 spontaneous abortion agarn and others surpass the previous abortion time . There are two still non - pregnancy 6 months after therapy ; We also found the level of TCRγδT cells , TCRγδ lymphocyte / CD3 cells are significantly higher after therapy than that of the before... |
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