| Objectives: Kiwifruit concentration, which content many bioactive components, are compounds from fresh kiwifruit . In this study we investigated whether concentration could provide protection to lipid peroxidation and promote the immune system and study the possible mechanisms.Methods: 1. The study of antioxidantion: 70 Wistar rats, aged 4 months, 250-260g bodyweight (half male and half female) were randomly divided into 5 groups, which of each is 14 rats. The first group was control; the second ,the third, the forth and the fifth groups were supplemented with different dose of kiwifruit concentration which are 5%, 10%, 15% and 30% respective. The period of the trial was about 30 days. At the end of the trial, the mice will be died without pain by drug anesthesia, and samples of the blood and tissues were collected for analysis. (1) Using the alkaline single cell gel electrophoresis technique(SCGE) to detect the oxidative DNA damage of lymphocytes induced by HjOz. The activities of superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px) , the content oflipofuscin (LPO) , malanydiadehyde (MDA)were measured by biochemical methods with the kits.(2)The oxidative damage of erythrocyte membrane was induced by hydroxyl free radical by Fenton reaction. Membrane fluidity was measured by the method of fluorescence polarization, and malanydiadehyde(MDA) ?superoxide dismutase(SOD) of erythrocyte membrane by biochemical methods. The antioxidative,anti-free radical and protective effects of concentration on biomembranes were observed.2. The study of promoting the immune function: 70 Kunming mice, aged 6-8 months, 18-22g Bodyweight (half male and half female) were randomly divided into 5 groups and number of each was 14 mice. The first group was control; the second, the third, the forth and the fifth group were supplemented with different dose of kiwifruit concentration which are 5%, 10%, 15% and 30%. The period of the trial is about 30 days. At the end of the trial, the mice will be killed without pain by drug anesthesia, and then samples of the blood and tissues were collected for analysis. Lymphocytes of mice spleen were cultured and the transformation was detected by the method of MTT. The phagocytosis of phagocytes was detected using the carbonic clearance and the content of immunoglobulin A(IgA) - immunoglobulin G(IgG), immunoglobulin M(IgM) in the serum by biochemical methods.Results: 1. The results of antioxidation: Compared with control group, the activities of SOD in supplement groups had been increased. The values of SOD were 266.34 NU/mL in control group, 287.53 NU/mL, 315.58 NU/mL, 324.38 NU/mL and 342.64 NU/mL in four groups with the supplementation of 5%, 10%, 15% and 30% respectively. There was a significant increase by 28% between 30% supplement group and control. The activitiesof GSH-Px were separately 236.76 and 257.83 in two groups of supplementation of 15% and 30% kiwifruit concentration. Compared with control, moreover the contents of MDA in serum of all supplemented groups were separately decreased by 25.2%, 32.1%, 35.4% and 38.8%. The contents of LPO in serum of all supplement groups were separately decreased by 4.8%, 7.8%, 20% and 49.6%, which of 15% and 30% supplement groups were more signigicant than others.The DNA oxidant damage of lymphocyte: Compared with control, the original damage was less in 30% supplemented group than other groups. The DNA damage induced by 5 umol/L H2C<2 was significantly less in groups with 10%, 15%, 30% supplementation than other two groups.Hydroxyl free radical induced by Fenton reaction could attack lipids from tissues or membrane. Compared with positive group, the level of MDA in the supplement groups decreased remarkably, activities of SOD obviously increased and the membrane fluidity was significantly improved and lipid peroxidation was inhibited. It was indicated that concentration had the protective effect on biomembranes and there was a dose-effect relationship between different doses of concentration.2. The result of promoting immune sy...
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