| Objective: Alzheimer's Disease (AD) is a chronic proceeding and spirit fail disease happening old age time preliminary it, which make the advanced function of the cerebrum cortex general damage. It plays an important role in senile disease register and is one of four disease resulting palalysis. But hitherto there is no perfect medication.Acupuncture the site can produce special conduction message in the JingLuo system, which had been tested, and the conduction message can extensively affect on other system in the body, especially can adjust the pathology body. According to JingLuo theory, the experiment tried to test that Electroacupunture (EA) can get good modulate function on AD through AD animal study, and through a series of correlative inspection and analyses to probe the mechanism about EA treating AD, at the same time, provide a reference for clinic treating AD.Methods: Let 60 SD female rats in the room temperature 3 days for adapting. And we divided them into Normal group and Model group at random: 12 rats in Normal group and 48 rats in Model group. Adopting the method ofinjecting AlCl3 into the rat's abdomen to duplicate old age dementia animal model. We begin to of the Model group 3 days before the experiment, quantity of drug separately is 0.2ml, 0.4ml, 0.6ml for adapting, 3 days later begin to inject with big dose of ALCl3 into the rat's abdomen with 49.4mg/kg in Model group. Distilled water 4ml/kg is injected into the rat's abdomen of normal group. In order to better absorbing, let the rats rest a day after continuous injection 3 days. Making model rats is total to 60 days. Carry on the quiz in maze afterwards making model finished, throwing away the die and the dubious rat. With the normal group rats' whole time of walking maze as the upper limit of 99% as the limit,the normal group rats is smaller than that value disusing. The surplus is randomly divided into Model group,EA group and Placetam group, per group 14 rats and adding the former normal group 10 rats, the experiment is beginning. EA group use EA treatment, the points are BaiHui, DaZhui, XinShu and ShenShu, using sparse and dense wave, frequency is 30 Hz, stimulate standard is the needle quivering and the rats can resist the stimulate, time is 10 min, once every two days. Placetam group rats douche Placetam (0.32g/kg) treating, per day is once, synchronously deal the same tieing with the EA group for 10 min the every other day. Normal group and Model group are fixd against the EA group equal means admittedly, 10 minutes each time, every other day once, Moreover not bringing to bear on whateverlyinterposes the step, Treat 40 days in all. Carrying on the action to all rats afterwards the test is over (maze experiment), for observing them memory and the cognition capability. The three days after treating finished, decollation to kill all rats, put the left brain tissue into 10% formaldehyde solution, the paraffin wax package covers up, then making the organisation cuts into slices, Acting HE dyeing and the immunity histochemistry examine. Shall the right side brain organisation rinse in the way of cold NS, depart hippocampi and cortex, making 10% tissue symmetrical plasm respectively, centrifugate and take the above clear liquid, freeze store in -20℃ awaiting check. Measure the MDA content change in the SD rats serum with the method of TBA compare color analysis, measure the SOD active change in the SD rats serum with the method of purine oxidation enzyme, measure the NO active change in the SD rats serum with the method of nitric acid deoxidize enzyme, measure the AcheE active change in the SD rats serum and brain tissue with the method of S-radicle compare color analysis, measure the ChaT active change in the SD rats brain tissue with the method of immunity histochemistry. Through compared with Placetam group, observe EA stimulate effect on AchE,ChaT,SOD activity and MDA,NO content in imitate dementia rats brain tissue, from the point of cholinergic neuro-transmitter and free radical, probe EA stimulate treatm... |
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