| Osteosarcoma (OS) is the most common primary malignant tumor of bone, comprising 20% of all such malignancies. Its biological behavior is always highly malignant. Its hematogenous metastasis occurs more frequently and early. Though clinic apply of high-dose chemotherapy makes the 5-year survival rate of OS climb to 80%, a significant percentage of patients die from lung metastasis. Growth factors (GFs) activate post-receptor way by combining special receptors, which is important to cells survival. The second messenger of most GFs is phosphatidylinositol 3, 4, 5-triphosphate (PIPS). PIPS can promote proliferation of cells by combining other kinases. Tumor suppressor phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a kind of protein tyrosine phosphatase. It can dephosphate PIPS into PIP2, reverse the use of PIPS and refrain cells proliferation induced by most GFs. Proliferation cell nuclear antigen (PCNA) is affiliated protein of DNA ploymerase 6 . It can be used to evaluate the state of cell proliferation. So far, there are few reports on the expression of PTEN, epidermal growth factor receptor (EGFR) gene in human OS.Objective: In order to evaluate the role of PCNA, PTEN and EGFR in OS as well as their interaction, their expression in OS is investigated. It is expected that the study will help us to elucidate the molecular mechanism of OS and provide theoretical foundation for genie therapy.Materials and methods: Thirty specimens of human OS tissues (OST) were obtained. They were classified as three grades based on the grade of differentiation (I grade 7 cases, II grade 14 cases, III grade 9 cases). The control groups were 10 casesosteochondroma tissues (OCT). Immunohistochemical S-P method was used to exam the expression of PCNA, PTEN and EGFR in OS. There was a significant difference when P<0.05. Results:1. Expression of PCNA protein: 1.1 The positive immunostaining rate of PCNA in OCT was 20.0%(2/10), in which negative and strong stain were 8, 2, respectively; The positive immunostaining rate in OST was 100.0 %( 30/30), in which negative and strong stain were 13, 17, respectively. Comparing the staining rate and intensity of OCT and OST respectively, there were significant differences (P<0.05). 1.2 In grade I of OST, PCNA weak and strong positive staining were 6, 1, respectively. In grade II+III of OST, PCNA weak and strong positive staining were 7, 16, respectively. Comparing the staining intensity, there was significant difference between grade I and grade II+III of OST. (P<0.05).2. Expression of PTEN protein: 2.1 In OCT, the positive immunostaining rate of PTEN was 90.0%(9/10), in which negative, weak, moderate and strong immunostaining were 1, 2, 2, 5, respectively. In OST, the positive immunostaining rate of PTEN was 70.0 %( 21/30), in which negative, weak, moderate and strong immunostaining were 9, 7, 12, 2, respectively. Comparing the staining rate, there was no significant difference (P>0.05). Comparing the staining intensity, there was significant difference (P<0.05). 2.2 Expression of PTEN protein in OST: 2.2.1 In grade I of OST, the positive immunostaining rate of PTEN was 85.7%(6/7), in which negative, weak and moderate immunostaining were 1, 3, 3, respectively. In grade II+III of OST, the positive immunostaining rate of PTEN was 65.2%(15/23), in which negative, weak, moderate and strong immunostaining were 9, 4, 9, 2, respectively. Comparing the staining rate and intensity, there were no significant differences (P>0.05). 2.2.2 In PCNA weak positive staining group, the positive immunostaining rate of PTEN was 76.9%(10/13), in which negative, weak, moderate and strong immunostaining were 3, 3, 6, 1, respectively. In PCNA strong positive staining group, the positive immunostaining rate of PTEN was 64.7 %(11/17), in which negative, weak, moderate and strong immunostainingwere 6, 4, 6, 1, respectively. Comparing the staining rate and intensity of PTEN, there were no significant differences (P>0.05).3. Expression of EGFR prot... |
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