Association Of Polymorphism In The Interleukin-4 Receptor Gene With Asthma

objective To investigate the association of polymorphism in interleukin-4 receptor gene with childhood asthma. Methods 30 unrelated asthmatic children were included as experimental groups, 30 normal children who excluded atopic diseases were served as control groups .Then the polymorphism of IL-4R Q576R were determined by PCR/RFLP and the level of blood serum total IgE was tested by ELISA. Result 1. the distribution frequency of heterozygous genotype Q576R and mutant allele R576 was significantly higher in asthmatic children than normal controls, P<0.05; 2. The total IgE level was significantly different between the asthmatic children and control groups , P<0.05 ; 3 the level of serum IgE between the two groups who carry the genotype Q576R and Q576Q was not significantly different P>0.05. Conclusion 1. The mutant R576 allele of IL-4R may be one of the candidate genes for susceptibility to asthma ; Fail to find allele R576 had effect on the total serum IgE level in asthmatic children.